Lipoprotein lipase as a new tool in steryl ester analysis

A new method for the quantitative hydrolysis of steryl esters is described. Using lipoprotein lipase (triacylglycero-protein acylhydrolase, EC 3.1.1.34), steryl esters are cleaved to yield free sterols and fatty acids. In comparison to alkaline hydrolysis, the method is more gentle, more accurate and less laborious. Quantification of the hydrolysis products was done with capillary gas chromatography. The practicability of the method is demonstrated for authentic steryl esters and steryl esters as they occur in needles of Scots pine (Pinus sylvestris L.).