Aurora-B激酶在肺腺癌组织中的表达及其临床意义

目的检测极光激酶B（Aurora-B激酶）在肺腺癌组织中的表达情况,探讨Aurora-B表达与肿瘤转移的关系。方法采用免疫组化SP法及Western blot分析47例肺腺癌组织标本[肺腺癌组,其中转移（转移组）15例、非转移（非转移组）32例]及11例正常肺组织标本（正常对照组）中Aurora-B的表达。结果肺腺癌组阳性表达率为63.8%（30/47）,明显高于正常对照组的9.1%（1/11）,差异有统计学意义（P〈0.05）;转移组阳性表达率为86.7%（13/15）,明显高于非转移组的53.1%（17/32）,差异有统计学意义（P〈0.05）。转移组、非转移组、正常对照组阳性着色细胞比例分别为（26.7±9.2）%、（15.3±6.4）%、（3.1±2.3）%;非转移组阳性着色细胞比例明显低于转移组（P〈0.05）,转移组、非转移组阳性着色细胞比例均明显高于正常对照组（均P〈0.05）。结论 Aurora-B激酶在肺腺癌组织中呈高表达,可能参与了肺腺癌的远端转移。

Expression of Aurora-B Kinase in Lung Cancer Tissues and Its Clinical Significance

Objective To detect the expression of Aurora-B kinase in lung cancer tissues and to probe into the potential correlation between Aurora-B expression and tumor metastasis.MethodsForty-seven cases with lung cancer were collected,among which there were 15 with distant metastasis and 32 without metastasis.Eleven cases of normal lung tissues were used as control.Immunohistochemical SP analysis and Western blot were used to detect the expression of Aurora-B kinase in both lung cancer tissues and normal lung tissues.Results Thirty in all 47（63.8%）cases of lung cancers positively expressed Aurora-B kinase,whereas only 1 in all 11（9.1%）normal lung cases positively expressed Aurora-B（P0.05）.And in 15 tumor cases with metastasis,13 positively expressed Aurora-B with a positive ratio as 86.7%;but in other 32 tumor cases without metastasis,only 17 positively expressed Aurora-B with a positive ratio as 53.1%（P0.05）.Immunohistochemical analysis showed that the average percentage of positive-stained cells was（26.7±9.2）% in tumor cases with metastasis,while those in tumor cases without metastasis and normal tissue were（15.3±6.4）% and（3.1±2.3）% respectively（P0.05）.Conclusion Aurora-B kinase has been found to overexpress in lung cancer and may be involved in tumor metastasis.