^99mTc标记抗心肌肌凝蛋白单克隆抗体在实验性心肌梗塞中的研究

为比较＾９９ｍＴｃ－ＡＭＬＡ的特异性，将两组心肌梗塞２４ｈ大鼠分别注射＾９９ｍＴｃ－ＡＭＬＡ（ｎ＝２４）和＾９９ｍＴｃ－Ｎ－ＩｇＧ（ｎ＝１８），为研究不同心肌梗塞时间对摄取抗体的影响，将另一组不同心肌梗塞时间（２ｈ到１４ｄ共８个亚组）的大鼠注射＾９９ｍＴｃ－ＡＭＬＡ，计算三组的ＩＤ％／ｇ和梗塞心肌摄取／正常心肌摄取比（ＩＭ／ＮＭ）用一只心肌梗塞的狗注射＾９９ｍＴｃ－ＡＭＬＡ后离体心肌显像，结果表明

99m~Tc labelled antimyosin monoclonal antibody in experimental myocardial infarction

The feasibility and specificity of imaging of myocardial infarct with 99mTc-AMLA was reported. The first group of myocardial infarction(MI) rats were injected 99mTc-AMLA at 24h post MI. The second group were injected with 99mTc-N-IgG for control. For study of the influence of MI onset to uptake of 99mTc-AMLA, the third group with varied duration of MI from 4h to 14d was used.ID%/g and Infracted/Normal uptake ratio(IM/NM) were calculated. One in vitroheart of MI dog was imaged at 24 h after injection of 99mTc-AMLA. The results showed that the infarcted myocardium uptake of 99mTc-AMLA was higher than the normal myocardium. The IM/NM reached 6.68 at 8h in the first group, while non-specific IM/NM of 99mTc-N-IgG was only 1.42 (P<0.01). There were no significant differences between the ratios of uptake of 99mTc-AMLA in any infarcted duration from 4h to 14d(P>0.05). The in vitro myocardium imaging showed that the localization of 99mTc-AMLA in myocardium was the same as the blank area of triphenyltetrazolium chloride staining. It was concluded that 99mTc-AMLA is a myocardial imaging agent specific to MI. Thereby, it can be used as a useful infarct avid imaging reagent.