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| 海带多糖纯化及清除自由基活性研究 | |
| 引用本文： | 海带多糖纯化及清除自由基活性研究[J]. 食品工业科技, 2013, (15): 80-83. DOI: 10.13386/j.issn1002-0306.2013.15.009 |
| 作者姓名： | 彭臻菲 方哲翔 刘敏 张其清 |
| 作者单位： | 1. 福州大学化学化工学院,福建福州350108;福州大学生物和医药技术研究院,福建福州350002 2. 福州大学生物和医药技术研究院,福建福州,350002 |
| 基金项目： | 福州大学科研启动基金，福建省科技重大专项专题 |
| 摘 要： | 海带多糖经S-300凝胶层析分离后得到纯化组分WPS-1-1。采用高效液相色谱、红外光谱对WPS-1-1结构进行解析,结果表明,WPS-1-1分子量为112ku,主要由岩藻糖、木糖、半乳糖和甘露糖构成,其摩尔比为6.9∶3.3∶1.5∶1。WPS-1-1含有β-吡喃糖苷键和硫酸基。体外自由基清除实验结果表明,WPS-1-1具有超氧阴离子自由基和羟自由基清除活性,其IC50分别为0.35、1.0mg/mL,可作为天然的功能性自由基高效清除剂。 |
| 关 键 词： | 海带多糖 清除自由基 单糖组成 |
| 收稿时间： | 2012-12-06 |
Purification and radical scavenging effect of Laminaria japonica polysaccharide |
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| Purification and radical scavenging effect of Laminaria japonica polysaccharide[J]. Science and Technology of Food Industry, 2013, (15): 80-83. DOI: 10.13386/j.issn1002-0306.2013.15.009 | |
| Abstract: | A purified polysaccharide of WPS-1-1 was obtained from crude extracts of Laminaria japonica by S-300 gel chromatography.The average molecular weight of WPS-1-1 was 112ku. Monosaccharides analysis revealed that WPS-1-1 was composed of fucose, xylose, galactose and mannose with a molar ratio of 6.9∶3.3∶1.5∶1. The results of infrared spectrum indicated that WPS-1-1 contained β-pyranose and sulfate group. In vitro, radical scavenging tests revealed that WPS-1-1 showed significant function of scavenging superoxide and hydroxyl radicals with IC 50 value of 0.35 and 1.0mg /mL.It could be a potential functional free-radical scavenger. |
| Keywords: | Laminaria japonica polysaccharide free radicals scavenging monosaccharide composition |
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