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Detection of Enterotoxigenic Escherichia coil in Foods by DNA Colony Hybridization |
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| Authors: | JOSEPH L FERREIRA WALTER E HILL MOSTAFA K HAMDY FRANCIS A ZAPATKA STEVEN G McCAY |
| Affiliation: | Authors Ferreira, Zapatka, and McCay are with the Science Division, Food &Drug Administration, Atlanta, GA 30309.;Author Hill is with the Division of Microbiology, Food &Drug Administration, Washington, DC20204.;Author Hamdy is with the Food Science Dept., Univ. of Georgia, Athens, GA 30602. |
| Abstract: | A DNA colony hybridization procedure was used to identify and enumerate a heat-labile toxin-producing strain of Escherichia coli (H10407) in various types of foods. Foods were seeded with H10407 cells and examined by DNA hybridization on nitrocellulose filters with 32P- labeled heat-labile toxin gene fragments. The number of cells recovered on plate count agar and eosine methylene blue agar was compared. With nitrocellulose filters, recoveries were about 81% on plate count agar and about 76% on eosine methylene blue agar. No significant differences in recovery were observed with ten different food types. |
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