| 新疆自然发酵酸马奶中酵母菌的分离鉴定 |
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| 引用本文： | 祝春梅,姚新奎,孟军,王建文,刘志安,刘佳,李智军,马军,任海帆.新疆自然发酵酸马奶中酵母菌的分离鉴定[J].食品与发酵工业,2013,39(4):42-47. |
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| 作者姓名： | 祝春梅 姚新奎 孟军 王建文 刘志安 刘佳 李智军 马军 任海帆 |
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| 作者单位： | 1. 新疆农业大学食品科学与药学学院,新疆乌鲁木齐,830052
2. 新疆农业大学动物科学学院,新疆乌鲁木齐,830052 |
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| 基金项目： | 国家农业部公益性行业专项"马驴产业技术研究与试验示范项目"，新疆维吾尔自治区科技重大专项"马(运动、肉用、乳用)生产关键技术研究与示范" |
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| 摘 要： | 从自然发酵酸马奶中分离出25株酵母菌,并对酵母菌进行传统形态学、生理生化特性鉴定。鉴定结果为:12株为克鲁维酵母属Kluyveromyces(8株为马克思克鲁维酵母Kluyveromyces marxianus,4株为乳酸克鲁维酵母Kluyveromyces lactis)、1株为酿酒酵母Saccharomyces cerevisiae、1株为白地霉Galactomyces geotrichum,其他菌株无法推断出。再利用5.8S rDNA序列同源性分析,对25株酵母菌进行分子生物学鉴定,同时对1、2、18、25、27、28号菌株进行系统发育树分析。鉴定结果为:10株单孢酿酒酵母Kazachstania unispora、8株马克思克鲁维酵母K.marxianus、4株乳酸克鲁维酵母K.lactis、1株酿酒酵母S.cerevisiae、1株白地霉G.geotrichum、菌株27疑似新种。
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| 关 键 词： | 酸马奶 酵母菌 分离鉴定 分子生物学鉴定 |
Isolation and identification of yeast form natural fermentation koumiss in Xinjiang |
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| Zhu Chun-mei,Yao Xin-kui,Meng Jun,Wang Jian-wen,Liu Zhi-an,Liu jia,;Li Zhi-jun,Ma Jun,Ren Hai-fan,Wei Li.Isolation and identification of yeast form natural fermentation koumiss in Xinjiang[J].Food and Fermentation Industries,2013,39(4):42-47. |
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| Authors: | Zhu Chun-mei Yao Xin-kui Meng Jun Wang Jian-wen Liu Zhi-an Liu jia ;Li Zhi-jun Ma Jun Ren Hai-fan Wei Li |
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| Affiliation: | 1(College of Food Science and Pharmaceutical Science,Xinjiang Agricultural University,Urumqi 830052,China) 2(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China) 3(Agricultural Technology Promotion Center,Qitai 831800,China) |
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| Abstract: | 25 strains of yeasts were isolated from the natural fermented koumiss according to the colonial morphology and biological properties.The results of identification were as follows: 8 strains of Kluyveromyces marxianus,4 strains of Kluyveromyces lactis,1 strain of Saccharomyces cerevisiae,and 1 strain of Galactomyces geotrichum,the rest can't be deduced.25 stain of yeasts were identified by 5.8S rDNA sequencing,and strain 1,2,18,25,27,and 28 were further identified by phylogenetic tree analysis.The results of identification were as follows: 10 strains of Kazachstania unispora,8 strains of Kluyveromyces marxianus,4 strains of Kluyveromyces lactis,1 strain of Saccharomyces cerevisiae,and 1 strains of Geotrichum candidum.Strain 27 might be a new species.The identification results were important for us to increase the awareness and usage of traditional fermented dairy products in yeast resources. |
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| Keywords: | koumiss yeast isolation and identification molecular biology identification |
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