大孔树脂对桑黄多糖提取液中色素的吸附与解吸特性研究

为了选择对桑黄多糖提取液纯化效果较好的树脂，比较了DA201等5种大孔吸附树脂对桑黄多糖提取液的脱色和除蛋白质效果．对5种树脂进行静态吸附与解吸试验，选择DA201树脂研究静态吸附动力学曲线及其解吸特性，考察树脂的饱和吸附量、脱色率、蛋白质脱除率及总糖的保留率．DA201树脂对桑黄多糖提取液中的蛋白质和色素的吸附较多，表明其脱蛋白和脱色效果较好，且多糖的回收率较高．对于特定的多糖提取液，流速1．0mL／min，过DA201树脂柱要使蛋白质和色素达到吸附饱和，需要连续进样10多个柱床体积（BV），蒸馏水和50％乙醇洗脱解吸效果均较好．DA201树脂较适于桑黄多糖提取液的初步纯化，为了充分发挥DA201树脂的吸附性能，工业生产上可考虑采用串柱法．

Adsorption and desorption characteristics of macroporous resins to pigments of Phellinus linteus polysaccharide extraction

In order to select a kind of resin which is better on purification of polysaccharide ex- tracted from the fruiting bodies of Phellinus Igniarius. Compared the effects of DA201 and other 5 kinds of macroporous resins in the proessing of decolorization and removal effects of liquid protein. On static adsorption and desorption experiments, select the DA201 resin for static adsorption kinetics and desorption characteristics, investigated the resin saturated ad- sorption, decolorization rate, protein removing rate and total sugar retention rate. The re- sults show. DA201 resin showed a good effects of deproteinization and decolorization, and total sugar retention rate was high. For the specific polysaccharide extraction, flow rate at 1.0 mL/min, DA201 resin column to make protein and pigment adsorption reached satura- tion, need continuous sampling 10 multiple column volume （BV） at least, distilled water and 50% ethanol elution desorption effects was suitable. DA201 resin was suitable for the deeolorization of polysaccharide,In order to make full use of DA201 resin adsorption proper- ties, the string column method can be considered in industrial production.