~(18)F-FDG-(OAc)_4在~(18)F-FDG中的残留及在小鼠体内的生物分布

为探讨~(18)F-FDG-(OAc)4在~(18)F-FDG中残留的原因及其与~(18)F-FDG在生物体内代谢分布的异同,本研究利用固相柱水解法制备~(18)F-FDG,研究NaOH用量、淋洗速率及淋洗液pH对~(18)F-FDG-(OAc)4残留的影响,以及~(18)F-FDG-(OAc)4在小鼠体内的代谢和分布规律。结果表明:NaOH不足是~(18)F-FDG-(OAc)4残留的主要原因,淋洗速率和淋洗液pH对其残留没有影响;注射~(18)F-FDG-(OAc)460min后,小鼠血、尿和脑代谢物中发现~(18)F-FDG-(OAc)4原形及部分水解物;小鼠体内分布结果显示,除肌肉外,~(18)F-FDG-(OAc)4和~(18)F-FDG在体内各脏器摄取值高低分布一致。

The 18F-FDG-(OAc)4 Residual in 18F-FDG and its Bio-distribution in Mice

To investigate the causes of 18 F-FDG-(OAc)4 residual in 18F-FDG and the discrepancy of bio-distributions between 18F-FDG-(OAc)4 and 18F-FDG,18F-FDG was prepared by hydrolysis on C-18.The effect of NaOH dosage,elution rate,and eluent pH on 18F-FDG-(OAc)4 residual,the metabolism and bio-distribution profiles of 18F-FDG-(OAe)4 in mice were stutied.The results showed that insufficiency of NaOH was the main reason for 18F-FDG-(OAc)4 residual,while the elution rate and the pH of the eluent posed no impact.After injecting 18F-FDG-(OAc)4 60 minutes,the partially hydrolyzed product and 18F-FDG-(OAc)4 were found in metabolite of blood、urine and brain of the mice.The mice bio-distribution suggested that the uptake of 18F-FDG-(OAc)4 and 18F-FDG by organs followed the same pattern apart from muscle.