| 高效液相色谱法测定虫草黄酒中虫草素的研究 |
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| 引用本文： | 孙然,冷云伟,胡奇杰,戚民,叶翔.高效液相色谱法测定虫草黄酒中虫草素的研究[J].徐州工程学院学报,2010(4):77-80. |
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| 作者姓名： | 孙然 冷云伟 胡奇杰 戚民 叶翔 |
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| 作者单位： | [1]中国矿业大学化工学院,江苏徐州221116 [2]湖州市质量监督检验所,浙江湖州313000 |
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| 基金项目： | 浙江省质量监督局计划项目（20100228） |
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| 摘 要： | 采用高效液相色谱法建立了虫草黄酒中虫草素含量的测定方法,探讨了虫草素提取条件,分析了流动相组成、柱温、波长对检测结果的影响.研究表明：色谱柱为Waters Symmetry C18柱,流动相为甲醇∶水=15∶85,流速为1mL/min;检测波长为288nm,柱温为40℃时分离、检测效果最好;方法检出限为0.05ng.该测定方法在黄酒质量控制方面具有应用价值.
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| 关 键 词： | 高效液相色谱法 虫草素 测定 |
Study of 3＇-Deoxyadenosine Yellow Wine Quality with High Performance Liquid Chromatography |
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| SUN Ran,LENG Yun-wei,HU Qi-jie,QI Min,YE Xiang.Study of 3＇-Deoxyadenosine Yellow Wine Quality with High Performance Liquid Chromatography[J].Journal of Xuzhou Istitute of Technology,2010(4):77-80. |
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| Authors: | SUN Ran LENG Yun-wei HU Qi-jie QI Min YE Xiang |
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| Affiliation: | 1.School of Chemical Engineering and Technology,China University of Mining and Technology,Xuzhou 221116,China;2.School of Quality Supervision and Inspection,Huzhou 313000,China) |
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| Abstract: | A method to determine 3＇-deoxyadenosine in Cordyceps sinensis yellow wine has been established with high performance liquid chromatography.The effect of several factors were studied,such as type of component of mobile phase,column temperature,detective wave length and the condition of extracting the 3＇-deoxyadenosine.The studies indicate that the effect of separation and determination are optimum when determination parameters are as follows：mobile phase：methanol/water=15/85;flow rate：1 mL/min;detective wave length：288 nm;column temperature：40 ℃.The detection limit is 0.05 ng.This detection method is of practical use in the quality control of yellow wine. |
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| Keywords: | HPLC Cordycepin determination |
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