Hydrosoluble Benzo[e]pyridoindolones as Potent Inhibitors of Aurora Kinases |
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Authors: | Ly‐Thuy‐Tram Le Dr Hong‐Lien Vu Delphine Naud‐Martin Marianne Bombled Dr Chi‐Hung Nguyen Dr Annie Molla |
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Affiliation: | 1. Biology Laboratory, CRI‐INSERM/UJF U823, Institut Albert Bonniot, Université Joseph Fourier, 38706 La?Tronche (France);2. Permanent address: DaNang University of Technology (Vietnam);3. Permanent address: Ho Chi Minh University of Natural Sciences (Vietnam);4. Chemistry Laboratory, UMR 176 CNRS, Institut Curie, Bat 110 Centre Universitaire, 91405 Orsay (France) |
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Abstract: | Aurora kinases play an essential role in mitotic progression and are potentially druggable targets in cancer therapy. We identified benzoe]pyridoindoles (BePI) as powerful aurora kinase inhibitors. Their efficiency was demonstrated both in enzymatic inhibition studies and in cell culture assays. New BePI molecules were synthesized, and a structure–activity relationship study was conducted with the aim of improving the activity and solubility of the lead compound. Tetracyclic BePI derivatives are characterized by a particular curved shape, and the presence of an oxo group on the pyridine ring was found to be required for aurora kinase B inhibition. New hydrosoluble benzoe]pyridoindolones were subsequently designed, and their efficacy was tested by a combination of cell‐cycle analysis and time‐lapse experiments in live cells. The most active BePI derivative, 13 b , inhibited the cell cycle, drove cells to polyploidy, and eventually induced apoptosis. It exhibited high antiproliferative activity in HeLa cells with an IC50 value of 63 nM . Relative to compounds tested in clinical trials, this antiproliferative potency places 13 b among the top 10 aurora kinase inhibitors. Our results justify further in vivo evaluation in preclinical animal models of cancer. |
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Keywords: | antitumor agents aurora kinases cancer inhibitors structure– activity relationships |
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