| 降烟碱细菌-烟草节杆菌K9的分离及鉴定 |
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| 引用本文： | 夏振远,雷丽萍,吴玉萍,郭荣君. 降烟碱细菌-烟草节杆菌K9的分离及鉴定[J]. 中国烟草科学, 2006, 27(2): 1-4 |
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| 作者姓名： | 夏振远 雷丽萍 吴玉萍 郭荣君 |
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| 作者单位： | 云南省烟草科学研究所,云南,玉溪,653100 |
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| 基金项目： | 国家自然科学基金;云南省烟草公司资助项目 |
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| 摘 要： | 采用烟碱为唯一碳源的选择性培养基,对烟草、植烟土壤及烟草污水中的细菌进行选择性分离,共分离得到97株能够分解利用烟碱的细菌,其中菌株K9分解能力最强。经生理生化分析以及16SrDNA序列同源性分析,鉴定为烟草节杆菌(Arthrobacternicotianae)。为微生物降烟碱的研究及应用提供了理论依据。
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| 关 键 词： | 烟碱 生物降解 鉴定 烟草节杆菌 |
| 文章编号： | 1007-5119(2006)02-0001-04 |
| 修稿时间： | 2006-04-03 |
Isolation and identification of degrading nicotine bacteria- Arthrobacter nicotianae strain K9 |
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| XIA Zhen-yuan,LEI Li-ping,WU Yu-ping,GUO Rong-jun. Isolation and identification of degrading nicotine bacteria- Arthrobacter nicotianae strain K9[J]. Chinese Tobacco Science, 2006, 27(2): 1-4 |
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| Authors: | XIA Zhen-yuan LEI Li-ping WU Yu-ping GUO Rong-jun |
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| Abstract: | 97 bacterial strains were separated and screened from tobacco,soil and sewage of tobacco factory with selectivity culture medium, in which nicotine was the sole carbon source. These strains could decompose and utilize nicotine,and strain K9 was best one that have strongly ability to degrade nicotine. Based on physiology and biochemistry characters and 16S rRNA gene sequence homology, the strain K9 was identified as Arthrobacter nicotianae. This study lays the foundation for the application of nicotine biodegradation. |
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| Keywords: | 16SrDNA |
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