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不同单菌制曲豆酱风味物质及氨基酸分析比较
引用本文:康蕾,胡茂丰,刘素纯.不同单菌制曲豆酱风味物质及氨基酸分析比较[J].中国酿造,2014(11):140-144.
作者姓名:康蕾  胡茂丰  刘素纯
作者单位:湖南农业大学食品科学技术学院;湖南农业大学生物科学技术学院;食品科学与生物技术湖南省重点实验室;
基金项目:湖南省科技计划项目(2012NK3072)
摘    要:分别采用米曲霉(A spergillusoryzae)A S3.042、高大毛霉(M ucorm ucedo)、根霉(R hizopus)和黑曲霉(A spergillusniger)制曲制酱,对后发酵黄豆酱中氨基酸态氮、风味物质和游离氨基酸进行测定分析,发现在45 d氨基酸态氮含量最高,在45~65 d时氨基酸态氮含量保持稳定;判定65 d为发酵终点。测得米曲霉65 d挥发性物质是4种酱中最丰富的,测定米曲霉豆酱游离氨基酸含量为4种酱中最多,为174.17 m g/g干质量,且种类最丰富。

关 键 词:黄豆酱  氨基酸组成  风味物质  固相微萃取  气质联用

Comparison of flavor compounds and amino acids of soybean paste fermented by different moulds
Affiliation:KANG Lei, HU Maofeng, LIU Suchun (1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2. College of Bioscience and Biotechnology, Hunch Agricultural University, Changsha 410128, China; 3.Hunan Key Laboratories of Food Science and Biotechnology, Changsha 410128, China)
Abstract:K ojiand soybean paste w ere m ade using A spergillus oryzae A S3.042, M ucor m ucedo,R hizopus and A spergillus niger as ferm entation starter,respectively.The am ino acid nitrogen,flavorcom pounds and free am ino acids contents ofthe products w ere determ ined and analyzed.The highestcontentofam ino nitrogen w asin 45 d,and am ino nitrogen rem ained stable from 45 d to 65 d.A tthe 65 d,the soybean paste ferm ented by A . oryzae had the m ostabundantvolatile com ponentsand am ino acids,and the free am ino acid contentw asthe highestof174.17 m g/g dry w eight.
Keywords:soybean paste  am ino acid com position  flavorcom ponents  solid-phase m icro-extraction  G C -M S
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