| 水产品及其制品中大西洋鲑鱼实时荧光PCR检测方法建立 |
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| 引用本文： | 王坤,林霖,吴佳辉,朱成杰,余灏,张世伟,杨国武.水产品及其制品中大西洋鲑鱼实时荧光PCR检测方法建立[J].食品科技,2021(1). |
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| 作者姓名： | 王坤 林霖 吴佳辉 朱成杰 余灏 张世伟 杨国武 |
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| 作者单位： | 深圳市计量质量检测研究院 |
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| 基金项目： | 深圳市食品安全战略项目(JSGG20140519114050315-7)。 |
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| 摘 要： | 目的:三文鱼营养丰富,含有ω-3不饱和脂肪酸,深受大众喜爱,但存在产品标签标示错误、掺伪掺假、以次充好的现象。目前采用检测标准SN/T 3589.7—2013《出口食品中常见鱼类及其制品的鉴伪方法第七部》对鲑鱼成分检测,无法区分大西洋鲑鱼和其他种类的三文鱼。因此有必要建立一种用于区分上述几种三文鱼的快速检测方法。方法:研究建立了针对大西洋鲑鱼成分鉴定的实时荧光PCR法,并对该方法的特异性和检出限进行了验证。结果:该方法具有良好的特异性,检出限为1%(w/w)。结论:可应用于大西洋鲑鱼的日常检测,为监管部门提供技术支持。
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| 关 键 词： | 大西洋鲑 属性鉴别 实时荧光PCR |
Detection Method Construction by Real-Time PCR for Salmosalar in Aquatic Product and Its Processed Products |
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| WANG Kun,LIN Lin,WU Jiahui,ZHU Chenjie,YU Hao,ZHANG Shiwei,YANG Guowu.Detection Method Construction by Real-Time PCR for Salmosalar in Aquatic Product and Its Processed Products[J].Food Science and Technology,2021(1). |
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| Authors: | WANG Kun LIN Lin WU Jiahui ZHU Chenjie YU Hao ZHANG Shiwei YANG Guowu |
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| Affiliation: | (Shenzhen Academy of Metrology&Quality Inspection,Shenzhen 518131,China) |
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| Abstract: | Objective:Salmon is rich in nutrition and contains omega-3 unsaturated fatty acids,which is popular among the public.However,there are some problems such as wrong labeling,adulteration and inferior quality.The current detection standard SN/T 3589.7—2013 can not distinguish Salmosalar from other salmon.It is necessary to establish a rapid detection method for distingnishing the above-mentioned types of salmon.Method:In this study,a real-time PCR method for the identification of Salmosalar was established,and its specificity and detection limit were verified.Result:It shows that the method has good specificity,detection limit is 1% (w/w).Conclusion:It can be applied to the daily detection of Salmosalar,provide technical support for the supervision department. |
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| Keywords: | Salmosalar adulteration discrimination real-time PCR |
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