Isolation and characterization of polygalacturonase and invertase from a yeast Endomycopsis fibuligera (3812) bioconversion of date wastes |
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| Authors: | SANAA T. EL-SAYED SORIAL A. MOHARIB ETIDAL W. JWANNY |
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| Affiliation: | N.R.C. Biochemistry Department, Tahrir Street, Dokki, Cairo, Egypt. |
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| Abstract: | Properties of polygalacturonase [E.C. 3.2.1.15] (E1) and invertase (E.C. 3.2.1.26] (E2) isolated and purified in powdered form from Endomycopsis fibuligera grown on date wastes were measured.
The molecular weights were estimated to be about 32000 and 69500, and they were highly specific for hydrolysing (1–4) and (1–2) glycosidic links respectively. the Km value of E1 was 7.1 × 10-2M for soluble-pectic acid and that for E2 was 6.7 × 10-2M for sucrose at pH4.5, and optimal pHs were 3.5 for E1 and 4.0 for E2. the purified enzymes are stable on storage for more than one year at - 20°C. |
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| Keywords: | solid state fermentation sugar degrading enzymes |
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