| 高粱DNA导入引起小麦HMW-GS的变异及其品质改良和变异机理分析 |
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| 引用本文： | 欧巧明,倪建福,崔文娟,庞斌双.高粱DNA导入引起小麦HMW-GS的变异及其品质改良和变异机理分析[J].中国粮油学报,2011,26(1):15-19. |
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| 作者姓名： | 欧巧明 倪建福 崔文娟 庞斌双 |
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| 作者单位： | 1. 甘肃省农业科学院生物技术研究所,兰州,730070
2. 北京市农林科学院杂交小麦工程技术研究中心,北京,100089 |
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| 基金项目： | 甘肃省重大科技专项基金(0801NKDA015); 甘肃省科技支撑计划[自然科学基金](0803RJZA041); 甘肃省农科院农业科技创新专项基金(2009GAAS16) |
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| 摘 要： | 采用花粉管通道法,将高粱(Sorghum bicolor L.)基因组DNA导入粉质籽粒的稳定品系89122中,后代经多代连续选择优良变异系,并进行了高分子质量麦谷蛋白亚基的SDS-PAGE电泳检测和品质化验分析。获得1个稳定遗传变异新品种,与导入受体89122相比,后代新品系2001502-23含有7+8、5+10优质亚基,HMW-GS发生明显变异,Gul D1位点基因发生等位变异,其表达产物由原来(89122)的2+12亚基为5+10亚基;其品质指标较受体得到改善,从而实现了受体小麦HMW-GS基因的遗传转化和品质改良。说明利用花粉管通道法完全可以实现小麦HMW-GS基因的转化和品质目标性状的遗传改良。而生物诱变是其可能的遗传转化和变异机理之一。
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| 关 键 词： | 小麦 高粱DNA 花粉管通道法 高分子质量谷蛋白亚基(HMW-GS) 等位变异 |
Introducing Sorghum DNA into Spring Wheat:HMW-GS Variation,Quality Improvement and Its Mechanisms |
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| Ou Qiaoming,Ni Jianfu,Cui Wenjuan,Pang Binshuang.Introducing Sorghum DNA into Spring Wheat:HMW-GS Variation,Quality Improvement and Its Mechanisms[J].Journal of the Chinese Cereals and Oils Association,2011,26(1):15-19. |
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| Authors: | Ou Qiaoming Ni Jianfu Cui Wenjuan Pang Binshuang |
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| Affiliation: | Ou Qiaoming1 Ni Jianfu1 Cui Wenjuan1 Pang Binshuang2(Bio-technology Institute,Gansu Academy of Agricultural Sciences1,Lanzhou 730070)(Hybrid Wheat Engineering And Technique Research Center,Beijing academy of agriculture andforestry sciences2,Beijing 100089) |
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| Abstract: | The genome DNA of sorghum was introduced into floury grain spring wheat No.89122 via the pollen tube pathway.Excellent variant lines were chosen with multi-generation selecting.The detection of HMW-GS by SDS-PAGE electrophoresis and quality analysis were conducted.One stable variant was obtained.Compared with its recipient No.89122,the new variant 2001502-23 contained quality subunits 7+8 and 5+10,significant variation of HMW-GS occurred,gene alleles appeared in Gul D1 Locus,and HMW-GS 5+10 were expressed i... |
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| Keywords: | wheat sorghum DNA pollen-tube pathway HMW-GS gene alleles |
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