| 大孔树脂吸附薯蔓黄酮条件优化及其清除氧自由基能力 |
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| 引用本文： | 李颖畅,马勇,樊严,刘丽萍.大孔树脂吸附薯蔓黄酮条件优化及其清除氧自由基能力[J].中国粮油学报,2011,26(2):103-107. |
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| 作者姓名： | 李颖畅 马勇 樊严 刘丽萍 |
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| 作者单位： | 1. 渤海大学生物与食品科学学院,锦州,121000
2. 锦州农业科学研究院生物技术所,锦州,121000 |
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| 摘 要： | 比较了7种大孔树脂对薯蔓黄酮的吸附和解吸效果,研究了HPD-600大孔树脂对薯蔓黄酮的纯化工艺参数及其对氧自由基的清除能力。结果表明,HPD-600大孔树脂是纯化薯蔓黄酮比较好的树脂;薯蔓黄酮在HPD-600树脂上的吸附平衡时间为3.5 h,解吸平衡时间为2.0 h;吸附的最适质量浓度为6.08 mg/L;解吸时宜选用体积分数60%乙醇溶液。该工艺生产的黄酮产品为黄色粉末,回收率为81.36%,纯度为77.58%。薯蔓黄酮具有清除氧自由基的能力,清除羟自由基的IC50为314.79μg/mL,清除超氧阴离子的IC50为326.92μg/mL。
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| 关 键 词： | 薯蔓黄酮 大孔树脂 吸附 氧自由基 |
Adsorption Optimization of Macroporous Resin to Flavonoids from Sweet Potato Vines and Scavening Ability to Oxygen Free Radicals |
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| Li Yingchang,Ma Yong,Fan Yang,Liu Liping.Adsorption Optimization of Macroporous Resin to Flavonoids from Sweet Potato Vines and Scavening Ability to Oxygen Free Radicals[J].Journal of the Chinese Cereals and Oils Association,2011,26(2):103-107. |
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| Authors: | Li Yingchang Ma Yong Fan Yang Liu Liping |
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| Affiliation: | Li Yingchang1 Ma Yong1 Fan Yan2 Liu Liping1(College of Biology and Food Science,Bohai University1,Jinzhou 121000)(Institute of Biological Technology,Jin Zhou Academy of Agricultural Science2,Jinzhou 121000) |
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| Abstract: | The characteristics of adsorption and desorption of seven kinds of macroporous resins to flavonoids from sweet potato vines were studied.The optimum industrial parameters of purifying the flavonoids by macroporous resin were determined.The scavenging ability on oxygen free radicals of the flavonoids was evaluated.Results:HPD-600 macroporous resin is the suitable resin for purifying flavonoids from sweet potato vines,adsorption equilibrium time is 3.5 h,desorption equilibrium time is 2.0 h,optimal concentrat... |
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| Keywords: | flavoniods from sweet potato vines macroporous resin adsorption oxygen free radicals |
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