Scanning holographic microscopy of three-dimensional fluorescent specimens |
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Authors: | Indebetouw Guy Zhong Wenwei |
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Affiliation: | Department of Physics, Virginia Tech, Blacksburg, Virginia 24061-0435, USA. gindebet@vt.edu |
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Abstract: | We demonstrate experimentally the three-dimensional reconstructions of fluorescent biological specimens using scanning holographic microscopy. Three-dimensional reconstructions with transverse resolution below about 1 microm of transmission and fluorescence emission images are presented and analyzed. The limitations of the method are discussed. |
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