| 油菜蜂花粉黄酮醇的测定及其抗氧化活性研究 |
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| 引用本文： | 杨佳林,孙丽萍,徐响,朱青.油菜蜂花粉黄酮醇的测定及其抗氧化活性研究[J].食品科学,2010(3). |
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| 作者姓名： | 杨佳林 孙丽萍 徐响 朱青 |
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| 作者单位： | 中国农业科学院蜜蜂研究所;北京市卫生局临床药学研究所; |
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| 基金项目： | 国家公益性行业(农业)科研专项经费项目(nyhyzx07-041) |
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| 摘 要： | 本实验通过盐酸酸解蜂花粉乙醇提取物,薄层色谱法分离酸解液中黄酮醇,并测定酸解液中黄酮醇的含量及其抗氧化活性。结果表明:蜂花粉乙醇提取物酸解后,槲皮素(Rf值0.59)含量为0.40mg/g,山奈酚(Rf值0.71)含量为3.98mg/g,酸解液DPPH.清除率强于花粉乙醇提取物。经制备液相分离,采用核磁和质谱鉴定出一种黄酮醇苷,其结构为山奈酚3-O-(2-O-β-D-吡喃葡萄糖)-β-D-葡萄糖。采用TLC-DPPH技术对蜂花粉乙醇提取物及其酸解液中抗氧化成分进行追踪,为花粉中黄酮醇苷的抗氧性研究提供参考。
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| 关 键 词： | 油菜蜂花粉 黄酮醇 抗氧化活性 |
Hydrolyzed Rape Bee Pollen Ethanol Extract: Qualitative and Quantitative Analysis of Flavonol and Antioxidant Activity Evaluation |
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| YANG Jia-lin,SUN Li-ping,XU Xiang,ZHU Qing.Hydrolyzed Rape Bee Pollen Ethanol Extract: Qualitative and Quantitative Analysis of Flavonol and Antioxidant Activity Evaluation[J].Food Science,2010(3). |
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| Authors: | YANG Jia-lin SUN Li-ping XU Xiang ZHU Qing |
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| Affiliation: | YANG Jia-lin1,SUN Li-ping1,XU Xiang1,ZHU Qing2(1.Bee Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100093,China,2.Beijing Municipal Health Bureau Clinical Pharmacy Institute,Beijing 100035,China) |
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| Abstract: | Ethanol(80% concentration) extract of rape pollen was hydrolyzed with hydrochloric acid and the resulting hydrolysate was analyzed for the qualification and quantitation of flavonol by thin layer chromatography(TLC),UV-Vis spectroscopy and HPLC,and was subjected to antioxidant activity evaluation.TLC results showed that bands with 0.59 and 0.71 of Rf value were quercetin and kaempferol,respectively.The contents of quercetin and kaempferol in the hydrolysate were 0.40 mg/g and 3.98 mg/g,respectively.The scav... |
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| Keywords: | rape pollen flavonol antioxidant activity |
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