| 枯草芽孢杆菌C3产抗菌物质发酵条件优化 |
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| 引用本文： | 杨永青,谢远红,张红星,熊利霞,马晓丹,刘慧,孔保华.枯草芽孢杆菌C3产抗菌物质发酵条件优化[J].中国酿造,2014(3):28-31. |
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| 作者姓名： | 杨永青 谢远红 张红星 熊利霞 马晓丹 刘慧 孔保华 |
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| 作者单位： | 北京农学院食品科学与工程学院;农产品有害微生物及农残安全检测与控制北京市重点实验室,食品质量与安全北京实验室;东北农业大学食品学院 |
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| 基金项目： | 国家“十二五”科技支撑计划项目(2012BAD28B02-01);“十二五”国家高技术研究发展计划(863)项目子课题(2012AA101606-05);北京市教育委员会面上项目(KM201410020010) |
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| 摘 要： | 利用筛选的枯草芽孢杆菌(Bacillus subtilis)C3研究提高产抗菌物质的发酵条件。通过测定枯草芽孢杆菌C3抗菌物质对黑曲霉孢子萌发的抑制率,设计6因素3水平正交试验得到优化的发酵条件为:种子液的菌龄12h,种子液接种量2%,发酵培养基装液量75mL/250mL,发酵培养基初始pH 7.0,发酵温度37℃,发酵时间48h。在优化发酵条件下,枯草芽孢杆菌C3产抗菌物质的抑菌活性比优化前提高了26.52%。
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| 关 键 词： | 枯草芽孢杆菌 抗菌物质 发酵条件优化 |
Optimization of fermentation conditions of antibacterial material production by Bacillus subtilis C3 |
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| YANG Yongqing;XIE Yuanhong;ZHANG Hongxing;XIONG Lixia;MA Xiaodan;LIU Hui;KONG Baohua.Optimization of fermentation conditions of antibacterial material production by Bacillus subtilis C3[J].China Brewing,2014(3):28-31. |
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| Authors: | YANG Yongqing;XIE Yuanhong;ZHANG Hongxing;XIONG Lixia;MA Xiaodan;LIU Hui;KONG Baohua |
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| Affiliation: | YANG Yongqing;XIE Yuanhong;ZHANG Hongxing;XIONG Lixia;MA Xiaodan;LIU Hui;KONG Baohua;College of Food Science and Engineering, Beijing University of Agriculture;Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Beijing Laboratory of Food Quality and Safety;College of Food Science, Northeast Agricultural University; |
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| Abstract: | The screened Bacillus subtilis C3 was used to study the fermentation conditions of antibacterial material production. By measuring antibac- terial material ofB. subtilis C3 on the inhibition rate of Aspergillus niger spore germination, an orthogonal experiment with 6 factors and 3 levels was conducted to optimize the fermentation condition. The optimum condition was follows： age of inoculums 12 h, inoculums 2%, fermentation medium with fluid volume 75 ml/250 ml, initial pH 7.0, temperature 37~C, fermentation time 48 h. Under the optimized condition, the activities of antibacteri- al material by B. subtilis C3 increased 26.52% comparing to the non-optimized one. |
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| Keywords: | Bacillus subtilis antibacterial material fermentation condition optimization |
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