Assessment of pulsed electric fields induced cellular damage in Saccharomyces cerevisiae: Change in performance of mitochondria and cellular enzymes |
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| Authors: | Wei Zhao Ruijin Yang Yan-jie Gu Yali Tang Chunyang Li |
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| Affiliation: | 1. State Key Laboratory of Food Science & Technology, Jiangnan University, No. 1800 Lihu Road, Wuxi 214122, China;2. School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Road, Wuxi 214122, China;3. Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling Street, Nanjing 210014, China |
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| Abstract: | Pulsed electric fields (PEF) induced cellular damage in Saccharomyces cerevisiae, mainly focusing on metabolic properties including performance of mitochondria and cellular enzymes, was evaluated in this study. The proportion of sublethally injured S. cerevisiae cells was around 90% after PEF treatment at 20 kV/cm for 100–500 μs. Sublethal injure S. cerevisiae correlated with damage to mitochondria and membranes caused by PEF, which was also confirmed by the glucose-energized repair process. An increase in plasma membrane Ca2+-ATPase, decrease of cytoplasmic esterase activity and changes of ten cellular enzymes of S. cerevisiae were observed after PEF treatment, suggesting the sublethal injury of S. cerevisiae is correlated with the inactivation or activation of cellular enzymes and aggregation of proteins under PEF treatment. |
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| Keywords: | Pulsed electric fields (PEF) Non-thermal processing Sublethal injury Cellular enzymes |
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