聚羟基脂肪酸酯/海藻酸钠纳米纤维的制备及其性能

针对聚羟基脂肪酸酯(P(3HB-co-4HB))和海藻酸钠(SA)常规情况下难共溶的问题,以P(3HB-co-4HB)为原料,SA为改性剂,三氯甲烷/水为溶剂,烷基糖苷(APG)为乳化剂,通过共混利用静电纺丝法制备了P(3HB-co-4HB)/SA纳米纤维膜。借助红外光谱仪、差示扫描量热仪、扫描电子显微镜表征P(3HB-co-4HB)/SA静电纺纳米纤维的分子间作用力、热性能和形貌;利用细胞毒性和细胞共培养测试表征了P(3HB-co-4HB)/SA纳米纤维的生物相容性。结果表明:P(3HB-co-4HB)/SA复合材料的玻璃化转变温度发生改变,当添加SA质量分数为6%时,静电纺纳米纤维具有均一的形貌,纳米纤维的平均直径为500 nm,孔隙率为74%,细胞毒性等级为0级,P(3HB-co-4HB)和SA具有良好的生物相容性。

Preparation and properties of polyhydroxy fatty acid ester/sodium alginate composite electrospun nanofibers

Aiming at the problem that polyhydroxy fatty acid ester (P(3HB-co-4HB)) and sodium alginate (SA) are difficult to co-solve under normal circumstances, a P(3HB-co-4HB)/SA nanofiber membrane were prepared using P(3HB-co-4HB) as raw material, SA as modifier, chloroform/water as solvent, alkyl glycoside as emulsifier. The intermolecular forces, thermal properties and morphology of P(3HB-co-4HB)/SA electrospinning nanofibers were characterized by infrared spectrometer, differential scanning calorimeter, and scanning electron microscope. The biocompatibility of P(3HB-co-4HB)/SA nanofibers was characterized by cytotoxicity test and cell co-culture. The results show that the formation of the P(3HB-co-4HB)/SA composite material caused changes in glass transition temperature. When the mass fraction of SA is 6%, the electrospinning nanofibers have a uniform morphology, and the average diameter of the nanofibers is 500 nm. It demonstrates a porosity of 74% and the grade 0 cytotoxicity, and the P(3HB-co-4HB))and SA has good biocompatibility.