树脂法分离纯化桑葚花色苷

分别对12种大孔吸附树脂和6种阳离子交换树脂对桑葚花色苷的吸附性能进行了比较,通过静态吸附和解吸实验筛选出最佳大孔吸附树脂为LX-68,最佳阳离子交换树脂为D001。分别对这2种树脂进行静态和动态条件优化,确定了LX-68树脂最佳纯化条件为:以吸光度值0.991,pH值为3的色素液,8BV/h上样,用pH值为2、体积分数为80%的酸性乙醇作洗脱剂,洗脱流速为1BV/h,纯化后色素色价为114,纯度为39.9%,花色苷收率为91.5%。D001树脂最佳纯化条件为:以吸光度1.411Abs,pH值为2的色素液,6BV/h上样,用pH值为1、60%的酸性乙醇以3BV/h的洗脱流速洗脱,得到色价为65的色素粉末产品,纯度为24.1%,花色苷收率为67.6%。LX-68树脂和D001树脂对桑葚花色苷均具有较好的吸附分离性能,且LX-68树脂的分离效果优于D001树脂。

Study on the Separation and Purification of Mulberry Anthocyanins by Resins

The static adsorption and desorption capacities of 12 kinds of macroporous adsorption resins and 6 kinds of cation exchange resins used for mulberry anthocyanins were compared.It was found that the best macroporous adsorption resin was LX-68 and the optimal cation exchange resin was D001on.Through the static and dynamic conditions optimization,the best purification conditions of mulberry red pigment used by LX-68 resin were: 0.991 Abs,pH 3,absorption velocity 8 BV/h,pH 2.and 80% of acidic ethanol as elution agents,elution velocity 1 BV/h.The pigment purified by LX-68 resin was a dark purple powder,the color value was 114.The anthocyanins content was 39.9%,and the recovery of anthocyanins was 91.5%.D001 resin's optimum purification conditions were: Abs of solution 1.411,pH 2 and 6 BV/h,and elution solvent pH 1,60% of acidic ethanol with 3 BV/h elution velocity.The pigment purified by D001 resin was a powder with color-value 65,anthocyanins content 24.1%,and the recovery of anthocyanins 67.6%.Both LX-68 resin and D001 resin presented good adsorption separation properties to mulberry anthocyanins.However,the LX-68 resin's separation effect was better than D001 resin.