| 蚯蚓脱氧核糖核酸酶的底物特异性及降解产物的特性 |
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| 引用本文： | 刘志贞,康慧芳,樊慧杰,王晓媛,杨利军,杨琦,牛勃.蚯蚓脱氧核糖核酸酶的底物特异性及降解产物的特性[J].粉末涂料与涂装,2008,21(5):356-359. |
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| 作者姓名： | 刘志贞 康慧芳 樊慧杰 王晓媛 杨利军 杨琦 牛勃 |
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| 作者单位： | [1]山西医科大学生物化学与分子生物学教研室,太原030001 [2]中国疾病预防控制中心,北京100050 [3]首都儿科研究所生物技术室,北京100020 |
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| 基金项目： | 国家自然科学基金
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山西省青年科研项目 |
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| 摘 要： | 目的研究蚯蚓组织中一种新型脱氧核糖核酸酶(EWD)的底物特异性及降解产物的特性。方法采用ZOR-BAX-Oligo柱-HPLC、琼脂糖凝胶电泳、紫外分光光度法等技术,分析EWD对环状质粒DNA、线状λ-DNA、大肠杆菌基因组的降解作用,及对真核鲱精DNA、单链DNA等的降解中间产物和终产物的特性,并观察其对RNA的降解作用。结果EWD对这几种DNA均有降解作用,降解产物为较均一的、小于18bp的寡核苷酸。但EWD对RNA无降解作用。结论EWD为脱氧核糖核酸内切酶类。
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| 关 键 词： | 蚯蚓 脱氧核糖核酸酶 底物 降解产物 |
| 文章编号： | 1004-5503(2008)05-0356-04 |
| 修稿时间： | 2007年10月9日 |
Substrate Specificity and Property of Degradation Product of A Novel Earthworm Deoxyribonuclease from Lumbricus bimastos |
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| Abstract: | Objective To study the specificity of substrate and property of degradation product of a novel earthworm deoxyribonuclease (EWD) of Lumbricus bimastos.Methods Analyze the degradation effect of EWD on circular plasmid DNA,linear λ-DNA and E.coli genome,as well as the properties of intermediate and final degradation products of eukaryotic DNA and single-stranded DNA,by a series of methods including ZORBAX-Qligo column chromatography-HPLC,agarose gel electrophoresis and ultraviolet spectrophotometry.The degradation effect of EWD on RNA was also observed.Results EWD showed degradation effect on all the above-mentioned DNA.The degradation product were even oligonucleotides at lengths of less than 18 bp.However,EWD showed no degradation effect on RNA.Conclusion EWD was a kind of DNase. |
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| Keywords: | Earthworm Deoxyribonuclease Substrate Degradation product |
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