| 牛初乳中免疫球蛋白G双抗夹心ELISA检测方法的建立 |
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| 引用本文： | 刘 金,王丽威,岳喜庆.牛初乳中免疫球蛋白G双抗夹心ELISA检测方法的建立[J].食品科学,2016,37(14):74-79. |
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| 作者姓名： | 刘 金 王丽威 岳喜庆 |
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| 作者单位： | 1.沈阳农业大学食品学院，辽宁 沈阳 110866；2.辽宁工程技术大学理学院，辽宁 阜新 123000 |
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| 基金项目： | 2011年度国家星火计划项目（2011GA650001） |
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| 摘 要： | 将牛免疫球蛋白G（immunoglobulin G，IgG）作为免疫原免疫BALB/c小鼠，通过细胞融合、筛选获得分泌抗牛IgG单克隆抗体的细胞株。制备小鼠腹水抗体，进一步纯化获得抗牛IgG单克隆抗体。建立双抗夹心酶联免疫吸附法检测牛初乳中IgG质量浓度，该方法在7.8～1 000 ng/mL范围内有良好的线性关系，最低检出限为7.06 ng/mL，批内变异系数为4.52%，批间变异系数为4.94%，回收率为91.85%～102.45%。此法操作简便、准确度高、稳定性好，可用于实际牛初乳样品的快速检测。
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| 关 键 词： | 牛初乳 免疫球蛋白G 单克隆抗体 双抗夹心酶联免疫吸附法 |
Development of Double Antibody Sandwich ELISA for Detection of Bovine Colostrum Immunoglobulin G |
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| LIU Jin,WANG Liwei,YUE Xiqing.Development of Double Antibody Sandwich ELISA for Detection of Bovine Colostrum Immunoglobulin G[J].Food Science,2016,37(14):74-79. |
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| Authors: | LIU Jin WANG Liwei YUE Xiqing |
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| Affiliation: | 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
2. College of Science, Liaoning Technology University, Fuxin 123000, China |
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| Abstract: | The hybridoma cells secreting monoclonal antibody (McAb) against bovine colostrum immunoglobulin G (IgG)
were developed by cell fusion technology after immunization of BALB/c mice with bovine IgG. McAbs were obtained by
purification of mouse ascites. A double antibody sandwich enzyme-linked immunosorbent assay (ELISA) technique was
established for the detection of bovine colostrum IgG. The linear range was 7.8–1 000 ng/mL and the limit of detection (LOD)
was 7.06 ng/mL. The intra-assay and inter-assay coefficient of variations were 4.52% and 4.94%, respectively. The recovery
rate was 91.85%–102.45%. The ELISA method can provide a simple, accurate and stabile approach for the rapid detection
of bovine colostrum IgG. |
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| Keywords: | bovine colostrum immunoglobulin G (IgG) monoclonal antibody sandwich ELISA |
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