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| 利用硅胶柱层析分离纯化平贝总碱的研究 | |
| 引用本文： | 翟爱华,王丽杰,张东杰,鲁明明. 利用硅胶柱层析分离纯化平贝总碱的研究[J]. 食品科学, 2009, 30(1): 119-121. DOI: 10.7506/spkx1002-6630-200901028 |
| 作者姓名： | 翟爱华 王丽杰 张东杰 鲁明明 |
| 作者单位： | 1.黑龙江八一农垦大学食品学院 2.锦州医学院畜牧兽医学院 3.大连富生制药有限公司 |
| 基金项目： | 黑龙江省科技攻关项目 |
| 摘 要： | 目的：对东北特产平贝母的生物碱进行分离纯化研究。方法：用硅胶柱层析法对平贝总碱进行分离纯化，固定相为薄层层析硅胶G，流动相为乙酸乙酯- 甲醇- 氨水(17:2:1)。用HPLC-ELSD 法对分离出的贝母素甲和贝母素乙的纯度进行测定。结果：分离出贝母素甲和贝母素乙，贝母素甲纯度为93.19%、贝母素乙纯度为92.09%。结论：采用的硅胶柱层析分离纯化可得到纯度较高的分离组分。 |
| 关 键 词： | 平贝总碱 硅胶柱层析 分离纯化 |
| 收稿时间： | 2008-04-06 |
Study on Separation and Purification of Total Alkaloids from Fritillaria ussuriensis Maxim by Silica Gel Column Chromatography |
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| ZHAI Ai-hua,WANG Li-jie,ZHANG Dong-jie,LU Ming-ming. Study on Separation and Purification of Total Alkaloids from Fritillaria ussuriensis Maxim by Silica Gel Column Chromatography[J]. Food Science, 2009, 30(1): 119-121. DOI: 10.7506/spkx1002-6630-200901028 | |
| Authors: | ZHAI Ai-hua WANG Li-jie ZHANG Dong-jie LU Ming-ming |
| Affiliation: | (1. College of Food Science, Heilongjiang August First Land Reclamation University, Daqing 163319, China；2. College of Animal Husbandry and Veterinary Medicine, Jinzhou Medical College, Jinzhou 121001, China；3. Dalian Fusheng Pharmaceutical Co. Ltd, Dalian 116600, China) |
| Abstract: | Silica gel column chromatography was used to separate and purify total alkaloids from Fritillaria ussuriensis Maxim. The solid phase was thin layer chromatography silica gel G and the mobile phase was ethyl acetate-methanol-ammonia (17:2:1). The purities of the separated products, pemine and peinine were determined using HPLC-ELSD. They are 93.19% and 92.09%, respectively. In conclusion, the separation method of silica gel column chromatography applied in this experiment can be used to gain highly purified products. |
| Keywords: | total alkaloids from Fritillaria ussuriensis Maxim silica gel column chromatography separation and purification |
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