Graphene-Au nanoparticle based electrochemical immunosensor for fish pathogen Aphanomyces invadans detection |
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Authors: | Xingpu Qi Tong Chen Daoli Lu |
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Affiliation: | 1. Jiangsu University, Zhenjiang, Jiangsu, China;2. Jiangsu Agri-animal Husbandry Vocational College, Taizhou, Jiangsu, P.R. China |
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Abstract: | Epizootic ulcerative syndrome (EUS) that is primarily caused by an invasive oomycete fungus Aphanomyces invadans is a devastating fish disease. Rapid diagnosis of EUS is significantly important for the control and treatment of this highly invasive disease. In our study, a label-free immunosensor constructed with G-AuNPs/SAM-Ab-BSA/GCE was proposed for the determination of Aphanomyces invadans. The electrode was prepared by the immobilization of anti-mycelium antibodies on graphene-AuNPs nanocomposite-cysteamine monolayers modified GCE. The optimized parameters were as follows: 90 min as the immersion time of SAM modified electrode in the anti-mycelium solution, 0.20 µg/mL as the concentration of anti-mycelium solution and 10 min as the interaction time of immunoreaction. The immunosensors exhibited low limit detection of 309 ng/mL and good reproducibility. |
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Keywords: | epizootic ulcerative syndrome Aphanomyces invadans graphene gold nanoparticles immunosensor |
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