| 芒果多糖的纯化与光谱分析 |
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| 引用本文： | 孙延芳,梁宗锁,张欣,Unger FRANK. 芒果多糖的纯化与光谱分析[J]. 食品科学, 2012, 33(7): 93-95. DOI: 10.7506/spkx1002-6630-201207020 |
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| 作者姓名： | 孙延芳 梁宗锁 张欣 Unger FRANK |
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| 作者单位： | 1.辽宁工程技术大学理学院2.西北农林科技大学生命科学学院3.西北农林科技大学食品科学与工程学院4.维也纳大学生物药理和药理技术实验室 |
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| 基金项目： | 中国科学院知识创新工程项目(KZCX2-XB2-05-01);“十一五”国家科技支撑计划项目(2008BAD98B08) |
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| 摘 要： | 采用热水浸提、乙醇沉淀法得到芒果多糖粗品；采用苯酚-硫酸法测其多糖含量为(46.3±1.75)mg/g。DEAE-25纤维素和Sephadex G-75凝胶层析柱进一步分离纯化得到白色絮状多糖；高效阴离子交换色谱法分析其单糖组成。结果表明：该多糖是由鼠李糖、半乳糖和葡萄糖等单糖组成，其含量分别为(6.3±1.40)、(11.9±1.76)mg/g和(13.8±1.52)mg/g。红外光谱分析表明具有明显的多糖特征吸收峰，该多糖结构主要为β-糖苷键连接的吡喃型葡聚糖。
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| 关 键 词： | 墨西哥芒果 多糖 高效阴离子交换色谱 红外光谱 |
Purification and Spectroscopic Analysis of Polysaccharide from Mango |
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| SUNYan-fang,LIANGZong-suo,ZHANGXin,UngerFRANK. Purification and Spectroscopic Analysis of Polysaccharide from Mango[J]. Food Science, 2012, 33(7): 93-95. DOI: 10.7506/spkx1002-6630-201207020 |
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| Authors: | SUNYan-fang LIANGZong-suo ZHANGXin UngerFRANK |
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| Affiliation: | (1. College of Science, Liaoning Technical University, Fuxin 123000, China；2. College of Life Science, Northwest A&F University,Yangling 712100, China；3. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China；4. Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna A-1090, Austria) |
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| Abstract: | Crude polysaccharide was extracted from Mexico mango using hot water extraction followed by ethanol precipitation.The polysaccharide concentration of crude extract was(46.3 ± 1.75) mg/g as determined by phenol-sulfuric acid assay.After further purification by DEAE-52 and Sephadex G-75 column chromatography,a white flocculent polysaccharide was obtained.High performance anion exchange chromatography with pulsed amperometric detection(HPAEC-PAD) was used to determine the monosaccharide composition.The results showed that the polysaccharide was composed of rhamnose,galactose and glucose with a ratio of(6.3±1.40),(11.9 ±1.76) mg/g and(13.8 ±1.52) mg/g,respectively.The purified polysaccharide exhibited the characteristic IR absorption of polysaccharide.The structure was pyranoid glucan with β-glycosidic bond. |
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| Keywords: | mango polysaccharide high performance anion exchange chromatography(HPAEC) infrared spectroscopy(IR) |
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