| 绿色木霉产纤维素酶菌种诱变 |
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| 引用本文： | 李忠玲,秦涛,岳淑宁,张红艳.绿色木霉产纤维素酶菌种诱变[J].中国酿造,2011(12). |
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| 作者姓名： | 李忠玲 秦涛 岳淑宁 张红艳 |
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| 作者单位： | 1. 陕西省科学院酶工程研究所,陕西西安,710600
2. 陕西省酶工程技术中心,陕西西安,710600 |
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| 基金项目： | 陕西省科学技术研究发展计划项目(2010K02-13) |
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| 摘 要： | 该文对绿色木霉进行了硫酸二乙脂(DES)诱变和微波诱变,结果表明,经硫酸二乙脂诱变得到DES8菌株FPA酶活力比出发菌株提高了27.67％,该菌株进行微波诱变得到WB8菌株FPA酶活力比DES8菌株提高了14.88％.与出发菌株相比,WB8菌株FPA酶活力提高了46.67％,CMC酶活力提高了42.37％.遗传稳定性研究表明,该菌株遗传性能稳定.
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| 关 键 词： | 微波 诱变 滤纸酶活 纤维素酶活 |
Mutation of cellulase-producing Trichoderma viride strain |
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| LI Zhongling
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QIN Tao
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YUE Shuning
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ZHANG Hongyan.Mutation of cellulase-producing Trichoderma viride strain[J].China Brewing,2011(12). |
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| Authors: | LI Zhongling QIN Tao YUE Shuning ZHANG Hongyan |
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| Affiliation: | LI Zhongling1,QIN Tao2,YUE Shuning1,ZHANG Hongyan1(1.Institute of Enzyme Engineering,Shaanxi Province Academy of Sciences,Xi'an 710600,China,2.Enzyme Engineering Technology Center of Shaanxi Province,Xian 710600,China) |
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| Abstract: | The cellulose-producing Trichoderma viride stain was mutated with diethyl sulfate(DES) and microwave.The results showed that the filter paper activity(FPA) of the strain DES8,which was mutated by DES,was 27.67% higher than that of original strain.And the FPA of the strain WB8,which was microwave mutated strain of DES8,was 14.88% higher than that of DES8.Compared with the original strain,FPA of strain WB8 was improved by 46.67% and the CMCase activity of WB8 was improved by 42.37%.Further study indicated tha... |
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| Keywords: | microwave mutation FPA cellulase activity |
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