| 日本血吸虫抗体电化学免疫传感器研究 |
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| 引用本文： | 青宪,楚霞.日本血吸虫抗体电化学免疫传感器研究[J].化学传感器,2008,28(2):46-50. |
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| 作者姓名： | 青宪 楚霞 |
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| 作者单位： | 1. 湖南师范大学化学化工学院,湖南长沙,410081
2. 湖南师范大学化学化工学院,湖南长沙410081;湖南大学化学与生物传感及化学计量学国家重点实验室,湖南长沙410082 |
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| 摘 要： | 该文研制了一种高灵敏的基于辣根过氧化物酶(HRP)放大的电化学免疫传感器用于日本血吸虫抗体的检测.实验采用夹心法的模式,在电极表面固定了日本血吸虫抗原(SjAg),用以捕获血清中的日本血吸虫抗体(SjAb),然后再与辣根过氧化物酶(HRP)标记的羊抗兔IgG二抗形成免疫复合物.用计时安培法检测HRP酶催化H2O2还原对苯二酚底物产牛的电流,其大小与日本血吸虫抗体(SjAb)浓度在一定范围内成正比.对免疫反应的实验条件如电极表面固定抗原的浓度,HRP酶标二抗的浓度进行了优化.此外,还考察了其它蛋白质对测定结果的影响.结果表明,该方法操作简单,灵敏度高,线性范围宽,检测下限低.
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| 关 键 词： | 日本血吸虫抗体(SjAb) 辣根过氧化物酶(HRP) 免疫传感器 |
Electrochemical immunosensor for the detection of Schistosoma japonicum antibody |
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| Qing Xian,Chu Xia.Electrochemical immunosensor for the detection of Schistosoma japonicum antibody[J].Chemical Sensors,2008,28(2):46-50. |
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| Authors: | Qing Xian Chu Xia |
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| Affiliation: | Qing Xian1,Chu Xia1,2 (1. Chemistry , Chemical Engineering College,Hunan Normal University,Changsha 410081,China ) ( 2. State Key Laboratory for Chem/Biosensing , Chemometrics,Hunan University,Changsha 410082,China) |
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| Abstract: | An electrochemical immunosensor based on horseradish peroxidase (H RP) has been proposed for the detection of Schistosoma Japonicum antibody (SjAb ) in rabbit serum. Schistosoma japonicum antigen (SjAg) was first immobilized on the gold electrode surface via glutaraldehyde crosslink, then incubated with the infected rabbit serum containing SjAb, and finally the goat anti-rabbit IgG labeled with horseradish peroxidase was sandwiched to form the immunocomplex on t he gold electrode surface. Assay conditions s... |
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| Keywords: | schistosoma japonicum antibody (SjAb) horseradish peroxidase (HRP) immunosensor |
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