| 一株降解呕吐毒素枯草芽孢杆菌的鉴定与降解效果研究 |
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| 引用本文： | 谭剑,杨硕,苏会波,吴延东,佟毅.一株降解呕吐毒素枯草芽孢杆菌的鉴定与降解效果研究[J].当代化工,2018(3):548-551. |
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| 作者姓名： | 谭剑 杨硕 苏会波 吴延东 佟毅 |
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| 作者单位： | 中粮营养健康研究院,国家能源生物液体燃料研发中心,北京 102209;营养健康与食品安全北京市重点实验室,北京 102209;老年营养食品研究北京市工程实验室,北京 102209
吉林中粮生化有限公司,玉米深加工国家工程研究中心,吉林 长春 130033 |
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| 基金项目： | 北京市科委未来科技城科研成果后补助项目，中粮生化专业化公司研发项目资助 |
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| 摘 要： | 从被污染的玉米中分离、纯化得到一株白色菌株,命名为NHIBC 006D,通过形态学观察和16S r DNA序列分析比对,初步鉴定为枯草芽孢杆菌(Bacillus subtilis)。通过发酵培养和呕吐毒素(Deoxynivalenol,DON)添加实验,发现该菌分泌了一种可有效降解呕吐毒素的胞外蛋白,最高降解率可达73.5%。同时,该菌株发酵液对玉米浆中的呕吐毒素降解率也达到80%左右,为该菌在发酵食品及饲料中呕吐毒素生物降解的应用提供了可能。
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| 关 键 词： | 呕吐毒素 枯草芽孢杆菌 鉴定 生物降解 Deoxynivalenol Bacillus subtilis Identification Biodegradation |
Identification of a Bacillus subtilis Strain With Deoxynivalenol Degradation Ability |
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| TAN Jian,YANG Shuo,SU Hui-bo,WU Yan-dong,TONG Yi.Identification of a Bacillus subtilis Strain With Deoxynivalenol Degradation Ability[J].Contemporary Chemical Industry,2018(3):548-551. |
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| Authors: | TAN Jian YANG Shuo SU Hui-bo WU Yan-dong TONG Yi |
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| Abstract: | A white strain was isolated from contaminated corn and purified on LB medium plates, and it was named NHIBC 006D. According to its morphological structure and 16S rDNA sequence analysis, the strain NHIBC 006D was identified as Bacillus subtilis.In further research by fermentation and adding DON,the strain was found to secrete a kind of extracellular protein which can degrade 73.5% of DON in 24 h. The strain was also able to degrade 70% of DON in corn pulp, providing the basis for degradation of DON in fermented food and feed. |
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