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Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering |
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| Authors: | Junichiro Marui Sumiko Ohashi-Kunihiro Tomohiro Ando Marie Nishimura Hideaki Koike Masayuki Machida |
| Affiliation: | 1 Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Higashi 1-1-1, AIST Tsukuba Central 6, Tsukuba 305-8566, Ibaraki, Japan;2 Plant–Microbe Interactions Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kan''non dai, Tsukuba, 305-8602, Ibaraki, Japan |
| Abstract: | Aspergillus oryzae penicillin biosynthetic genes were clustered. The penicillin production was positively regulated by VeA, a global gene regulator required for transcriptional expression of the penicillin biosynthetic genes. Overexpression of the biosynthetic genes by a strong promoter yielded a greater than 100-fold increase in penicillin production. |
| Keywords: | Aspergillus oryzae Secondary metabolism Penicillin VeA Genetic engineering |
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