| 基于主成分分析法和响应曲面法对Halomonas菌株生长因子的分析与优化 |
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| 引用本文： | 史可,黄国富,徐化春,薛建良,孙静宽,肖新峰,李琳.基于主成分分析法和响应曲面法对Halomonas菌株生长因子的分析与优化[J].中国炼油与石油化工,2019,21(4):81-87. |
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| 作者姓名： | 史可 黄国富 徐化春 薛建良 孙静宽 肖新峰 李琳 |
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| 作者单位： | 1. 山东科技大学化学与环境工程学院;2. 潍坊科技学院;3. 黄河三角洲山东省生态环境科学重点实验室; |
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| 摘 要： | The biomass of petroleum-degrading bacteria, such as Halomonas spp., is crucial to the alleviation of severe oil spills through bioremediation. In this paper, the bacterium (HDMP1) was isolated and identified. Growth factors were analysed and optimised through the single-factor experiments, the factor analysis (FA), the principal component analysis (PCA), and the response surface methodology (RSM). Results indicated that HDMP1 was identified as genus Halomonas. In the single-factor experiments, the range of suitable growth conditions for HDMP1 covered: a salt concentration of 2%-4%, a medium pH value of approximately 9, an inoculum concentration of 1.0%, a substrate concentration of 1.0%-1.4%, and a rotation rate of 140 r/min. The evaluation by FA and PCA indicated that three significant growth factors were the salt concentration, the pH value, and the rotation rate. A maximum biomass of HDMP1 was obtained under the conditions covering a salt concentration of 3.5%, a medium pH of 8, and a rotation rate of 151 r/min by optimization.
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| 关 键 词： | 生物量 因子分析 主成分分析 生长因子 响应曲面法 |
| 收稿时间： | 2019-01-28 |
| 修稿时间： | 2019-02-24 |
Analysis and Optimisation of Halomonas Growth Factors Based on PCA and RSM |
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| Ke Shi Guofu Huang Huachun Xu Jian-liang XUE Jingkuan Sun Xinfeng Xiao Lin Li.Analysis and Optimisation of Halomonas Growth Factors Based on PCA and RSM[J].China Petroleum Processing and Petrochemical Technology,2019,21(4):81-87. |
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| Authors: | Ke Shi Guofu Huang Huachun Xu Jian-liang XUE Jingkuan Sun Xinfeng Xiao Lin Li |
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| Affiliation: | 1. College of Chemical and Environmental Engineering,Shandong University of Science and Technology;2. Weifang University of Science and Technology;3. Shandong Key Laboratory of Eco-Environmental Science for Yellow River Delta（Binzhou University）; |
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| Abstract: | The biomass of petroleum-degrading bacteria, such as Halomonas spp., is crucial to the alleviation of severe oil spills through bioremediation. In this paper, the bacterium (HDMP1) was isolated and identified. Growth factors were analysed and optimised through the single-factor experiments, the factor analysis (FA), the principal component analysis (PCA), and the response surface methodology (RSM). Results indicated that HDMP1 was identified as genus Halomonas. In the single-factor experiments, the range of suitable growth conditions for HDMP1 covered: a salt concentration of 2%-4%, a medium pH value of approximately 9, an inoculum concentration of 1.0%, a substrate concentration of 1.0%-1.4%, and a rotation rate of 140 r/min. The evaluation by FA and PCA indicated that three significant growth factors were the salt concentration, the pH value, and the rotation rate. A maximum biomass of HDMP1 was obtained under the conditions covering a salt concentration of 3.5%, a medium pH of 8, and a rotation rate of 151 r/min by optimization. |
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| Keywords: | biomass factor analysis principal component analysis growth indicators response surface methodology |
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