紫草细胞悬浮培养的结构化动力学模型

引言 植物细胞培养技术已经历了近40年的发展,但目前对其培养动力学的数学描述还比较缺乏.基于细胞内多组分变化及其相互作用的结构模型能较精确的描述细胞的培养过程,如Bailey和Nicholson3]提出的对数-线性模型,Frazie2]提出的"渗漏模型",Hooker3]提出的结构化模型等.但由于其形式复杂,不可测或待测参数太多,实际应用很少.

STRUCTURED KINETIC MODEL OF LITHOSPERMUM ERYTHRORHIZON CELL SUSPENSION CULTURE

By suspension cultivating of Lithospermum erythrorhizon and determining the dynamic curves, a structural kinetic model was established. By using the equation sets including 14 parameters and 9 state variables,the changes of excellular carbon source, intracellular carbon source, intracellular structural components,intracellular intermediate,cell respiration loss and intra/extracellular products were discussed. The model parameters were estimated by the Runge-Kutta method and the nonlinear Powell method. The sensitivity analysis was done. The simulation results could fit the real curves quite well. It was the first time to use a structural model to describe the suspension culture dynamics of Lithospermum erythrorhizon cells. The structural model was also the foundation for further study.