He-Ne激光和增强UV-B辐射对小麦幼苗根细胞凋亡的影响

采用5mW/mm2 He-Ne激光辐照、10.08kJ/(m2.d)UV-B辐射及二者组合对冬小麦"临优2018"幼苗进行处理,研究各处理组在不同处理天数下细胞凋亡的变化。研究结果显示,增强UV-B辐射能诱导小麦幼苗根尖出现细胞核向中心聚集,并形成凋亡小体等细胞凋亡现象。用流式细胞仪对不同处理组细胞凋亡情况进行定量分析,结果同样证明了增强UV-B处理能增加凋亡的细胞数目,并且在处理第5天时变化最为明显。而经He-Ne激光和UV-B复合处理后,细胞凋亡数目比单独UV-B处理组明显减少,差异极显著。因此认为He-Ne激光在一定程度上缓解了增强UV-B辐射对小麦幼苗根细胞凋亡的诱导作用。

Effect of He-Ne Laser and UV-B Radiation Enhancement on Apoptosis in the Roots of Wheat Seedling

Through the influence of the He-Ne laser irradiation of 5 mW/mm2 He-Ne, 10.08 kJ/(m2·d) UV-B and the combination of the two on the seeding of winter wheat "Linyou 2018", the modification of apoptosis on the roots of seedling in different treatment days and each group are analyzed. The research results indicate that, the features of apoptosis are found in the roots of wheat seedling after enhanced UV-B irradiation, such as nuclear assembled, apoptopic bodies. Apoptosis in different treatment groups by flow cytometry are quantitative analyzed. The results also show that the number of apoptosis are increased after enhanced UV-B irradiation and the differences are obvious in the 5 th day. With the management of He-Ne laser after UV-B radiation, the number of apoptosis is less than that of the UV-B treatment group and decreases obviously. Therefore, the apoptosis of roots of wheat seedling induced by enhanced UV-B radiation can be remited partly by He-Ne laser irradiation.