| 黑龙江省大豆灰斑病菌遗传多样性 |
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| 引用本文： | 孙洪利,刘洋大川,张俊华,韩英鹏,李文滨.黑龙江省大豆灰斑病菌遗传多样性[J].中国油料,2011(1):57-61. |
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| 作者姓名： | 孙洪利 刘洋大川 张俊华 韩英鹏 李文滨 |
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| 作者单位： | [1]东北农业大学农学院,黑龙江哈尔滨150030 [2]东北农业大学大豆研究所大豆生物学教育部重点实验室,黑龙江哈尔滨150030 |
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| 基金项目： | 黑龙江省博士后基金项目（LBH-Z06164）; 大豆生物学省部共建教育部重点实验室开放课题 |
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| 摘 要： | 从100对AFLP引物组合中筛选出多态性高且稳定性好的10对引物组合,对2006-2009年采自黑龙江省各大豆主要产区的121个大豆灰斑病菌菌株进行AFLP分析,得到148个多态性条带。用UPGMA聚类分析,121个菌株被归类于111个不同单元型（带型）,以彼此间的遗传距离小于0.81为界,将111个单元型划分为6个遗传系谱。其中L03、L05和L06为黑龙江大豆灰斑病的优势系谱。L02分布地域比较狭窄,为黑龙江大豆灰斑病的稀有遗传系谱。
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| 关 键 词： | 大豆灰斑病 扩增片段长度多态性 遗传多样性 大豆灰斑病菌 |
Genetic diversity of Cercospora sojina of soybean in Heilongjiang province |
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| Authors: | SUN Hong-li LIU YANG Da-chuan ZHANG Jun-hua HAN Ying-peng LI Wen-bin |
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| Affiliation: | 1.College of Agronomy,Northeast Agricultural University,Harbin 150030,China; 2.Soybean Research Institute,Northeast Agricultural University;Key Laboratory of Soybean Biology of Education Ministry,Harbin 150030,China) |
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| Abstract: | Ten polymorphic primer pairs were selected from 100 pairs of AFLP primers by using DNA of Cercospora sojina.A total of 148 polymorphic fragments were obtained by analyzing the 121 C.sojina isolates obtained in Heilongjiang province from 2006 to 2009.111 halotypes were identified by cluster analysis of the fingerprints of 121 C.sojina isolates using UPGMA software.At the genetic distance threshold 0.81,six genetic lineages were identified in the 111 haplotypes.The linages L03,L05 and L06 were dominant in Heilongjiang province,whereas the linage L02 rarely occured in this province. |
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| Keywords: | Soybean frogeye disease AFLP Genetic diversity Cercospora sojina |
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