Lipase production by solid fermentation of soybean meal with different supplements |
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Authors: | Elisandra Rigo Marco Di Luccio André Eliezer Polloni Francieli Arbter Débora de Oliveira |
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Affiliation: | a Departamento de Engenharia Química e de Alimentos, Universidade Federal de Santa Catarina, UFSC, SC, Brazil b Universidade Regional Integrada do Alto Uruguai e das Missões, Programa de Pós-Graduação em Engenharia de Alimentos, Food Engineering, Campus de Erechim, Av. Sete de Setembro, 1621, 99700-000, Erechim, RS, Brazil |
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Abstract: | The aim of this work was to study the production of extracellular lipases by solid state fermentation in soybean meal with different supplements. Lipase production by two microorganisms, screened by their potential for lipase production, was followed in terms of hydrolytic activity at different pH values(4, 7 and 9). The supplementation of the medium with urea and soybean oil significantly increased the enzyme production for all studied pH and microorganisms. Microorganism Penicillium P58 and P74 showed the possibility of production of different lipases with alkaline and acidic characteristics. In soybean meal supplemented with urea and soybean oil, this microorganism yielded 139.2 and 140.7U lipase/g of dry substrate in 48 h of fermentation, in alkaline and acidic conditions, respectively. Different behavior was observed when the enzyme extract was evaluated in neutral conditions, which yielded 180.0U lipase/g in 72 h. A new promising lipase producer strain was testedon soybean meal with different supplements. The strain produced a lipase with high activities by solid state fermentation of soybean meal supplemented with urea and soybean oil. |
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Keywords: | Lipase Penicillium sp Solid state fermentation Soybean meal |
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