| 大豆24kDa油体蛋白与人bFGF融合基因转化拟南芥的研究 |
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| 引用本文： | 薛萍,庞实锋,李洪志,李营,柳月英,于勤明,熊丽冬,李海燕,李校堃. 大豆24kDa油体蛋白与人bFGF融合基因转化拟南芥的研究[J]. 中国油料, 2011, 0(4): 344-350 |
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| 作者姓名： | 薛萍 庞实锋 李洪志 李营 柳月英 于勤明 熊丽冬 李海燕 李校堃 |
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| 作者单位： | [1]吉林农业大学生物反应器与药物开发教育部工程研究中心,吉林长春130118 [2]吉林农业大学生命科学学院,吉林长春130118 [3]广东医学院生物教研室,广东东莞523808 [4]牡丹江医学院医药研究中心,黑龙江牡丹江157011 [5]吉林市农业科学院,吉林吉林132012 |
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| 基金项目： | 国家863计划（2007AA100503）; 教育部科技创新工程重大项目培育资金（70S018）; 吉林省科技发展计划（20070922）; 长春市科技计划（06GG150） |
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| 摘 要： | 为探索大豆油体蛋白与人bFGF（碱性成纤维细胞生长因子）蛋白在植物油体中融合表达的可行性,以大豆总DNA为模板,通过PCR扩增得到大豆油体蛋白基因（Ddoil）及启动子（Ddprm）,构建Ddprm启动的Ddoil基因与植物偏好密码子改造的bFGF基因融合表达载体p1390Ddprm-Ddoil-bFGF,花侵染法转化拟南芥,对T2拟南芥抗性植株基因组进行PCR检测,对种子总蛋白进行SDS-PAGE分析、Western blot检测、ELISA检测及细胞活性测定,为bFGF在其他油料作物的油体系统的表达提供参考。
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| 关 键 词： | 大豆油体蛋白 碱性成纤维细胞生长因子（bFGF） 拟南芥 |
Soybean 24kDa oleosin and human bFGF fusion gene transformation and expression in Arabidopsis thaliana |
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| XUE Ping,PANG Shi-feng,LI Hong-zhi,LI Ying,LIU Yue-ying,YU Qin-ming,XIONG Li-dong,LI Hai-yan,LI Xiao-. Soybean 24kDa oleosin and human bFGF fusion gene transformation and expression in Arabidopsis thaliana[J]. , 2011, 0(4): 344-350 |
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| Authors: | XUE Ping PANG Shi-feng LI Hong-zhi LI Ying LIU Yue-ying YU Qin-ming XIONG Li-dong LI Hai-yan LI Xiao- |
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| Affiliation: | kun,(1.Engineering Research Center of Bioreactor and Pharmaceutical Development of the Ministry of Education,Changchun 130118,China; 2.College of Life Science,Jilin Agricultural University,Changchun 130118,China; 3.Department of Biology,Guangdong Medical College,Dongguan 523808,China; 4.Medicine and Pharmacon Research Center of Mudanjiang Medical University,Mudanjiang 157001,China; 5.Jilin Academy of Agricultural Sciences,Jilin 132012,China) |
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| Abstract: | In order to evaluate the expression of oleosin-bFGF fusion protein in plant oil body,polymerase chain reaction amplification was used to clone a soybean oleosin promoter Ddprm,a soybean oleosin gene（Ddoil） and a bFGF gene which was changed with plant codon usage.All these were used to construct a plant binary expression vector p1390 Ddprm-Ddoil-bFGF,and Agrobacterium-mediated transformation of Arabidopsis thaliana was conducted by floral dip.The results showed that bFGF gene integrated into Arabidopsis genome.The total protein of the T2 seeds were extracted for SDS-PAGE,Western blot,ELISA and cell proliferation activity to further assess the oleosin-bFGF fusion protein. |
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| Keywords: | Soybean oleosin Basic fibroblast growth factor（bFGF） Arabidopsis thaliana |
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