| 利用酰胺活化聚乙烯醇作为载体固定漆酶的研究 |
| |
| 引用本文： | 刁颖辉,付时雨,余惠生,王秋玲.利用酰胺活化聚乙烯醇作为载体固定漆酶的研究[J].纤维素科学与技术,2002,10(3):11-15,30. |
| |
| 作者姓名： | 刁颖辉 付时雨 余惠生 王秋玲 |
| |
| 作者单位： | 中国科学院广州化学研究所纤维素化学重点实验室,广州,510650 |
| |
| 基金项目： | 国家自然科学基金，广东省自然科学基金资助课题 |
| |
| 摘 要： | 交联聚乙烯醇经羧基化、酰胺化制成一种活性固定化酶的载体,并在温和的条件下对漆酶进行了固定。比较不同的固定化时间,pH值,温度和离子强度对固定化效果的影响,发现在12 h,40℃,pH值为3.2所制得的固定化酶的活力最高;在0.05~1.0 mol/L的范围内,随着作为固定化反应介质的缓冲溶液浓度的增加,所制得的固定化酶的活力有所下降。还发现固定化酶较游离酶的酶催化反应最适pH值有所升高。
|
| 关 键 词： | 漆酶 聚乙烯醇 固定化酶 |
| 文章编号： | 1004-8405(2002)03-0011-06 |
| 修稿时间： | 2002年4月3日 |
Study of Immobilization of Panus conchatus Laccase on the Amide-Activated Polyvinyl Alcohol |
| |
| Diao Yinghui,Fu Shiyu,Yu Huisheng,Wang Qiuling.Study of Immobilization of Panus conchatus Laccase on the Amide-Activated Polyvinyl Alcohol[J].Journal of Cellulose Science and Technology,2002,10(3):11-15,30. |
| |
| Authors: | Diao Yinghui Fu Shiyu Yu Huisheng Wang Qiuling |
| |
| Abstract: | Amide-activated polyvinyl alcohol (PVA) could be used as a carrier for the immobilization of Panus conchatus laccase. The effects of immobilization conditions such as reaction temperature, time, pH, and ion strength on the activity of immobilized laccase were studied. It was found that the optimal immobilization conditions were 12 h reaction time, 40℃ and pH 3.2 . It was also found that the activity of immobilized laccase decreased with the increase of buffer concentration in the range of 0.05~1.0 mol/L. Additionally, the optimal pH of immobilized laccase was higher than that of free laccase. |
| |
| Keywords: | laccase polyvinyl alcohol immobilized enzyme |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
