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Characterization and composition of sterols in the free and esterified sterol fractions ofAspergillus oryzae
Authors:Yasuhiko Fujino  Masao Ohnishi
Affiliation:(1) Department of Agricultural Chemistry, Obihiro University, Obihiro, Hokkaido, Japan
Abstract:Free and esterified sterol fractions were isolated fromAspergillus oryzae, and their components analyzed mainly by gas chromatography-mass spectrometry. Cholesterol, brassicasterol, episterol (5α-ergosta-7,2428]-dien-3β-ol), 4α-methyl-5α-ergosta-89],2428]-dien-3β-ol, lanosterol and 24-methylene-24-dihydrolanosterol were well characterized, whereas 5-dihydroergosterol, sitosterol and 2428]-dehydroergosterol were tentatively characterized. The principal sterol of the free sterol fraction was brassicasterol, and that of the esterified sterol fraction was episterol. Ergosterol, which is reported to be widely distributed in the fungi kingdom, was not detected.
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