碳离子全身辐照对小鼠血清及肝脏中超氧化物歧化酶活性、丙二醛含量和脑组织中还原性谷胱甘肽浓度的影响

用12C6 离子束对小鼠进行吸收剂量分别为0.05、0.1、0.3、0.5、0.75、1、1.5、2Gy的一次性全身照射,5d后测定血清及肝脏中超氧化物歧化酶(Superoxide dismutase,SOD)活性和丙二醛(Maleic dialdehyde,MDA)含量,以及脑组织中还原性谷胱甘肽(Glutathione,GSH)的浓度.结果显示,吸收剂量小于0.75 Gy小鼠的血清及肝脏中SOD活性高于对照组,大于0.75 Gy则低于对照组;吸收剂量小于0.3 Gy小鼠的血清及肝脏中MDA含量小于对照组,大于0.3 Gy则大于对照组;吸收剂量小于0.5 Gy小鼠的脑组织GSH浓度大于对照组,大于0.5 Gy则小于对照组.低剂量的重离子全身辐照对小鼠的抗氧化系统有一定的激活作用,随着照射剂量的增大,抗氧化酶活性明显降低,脂质过氧化水平增高.

Superoxide dismutase activity and maleic dialdehyde content in serum and liver and glutathione level in brain of 12C6+ ions-irradiated mice

Mice were irradiated by 831MeV 12C6 ions to 0.05, 0.1, 0.3, 0.5, 0.75, 1, 1.5, 2.0Gy, respectively. On 5th day after the irradiation, (Superoxide dismutase, SOD) activity and (Maleic dialdehyde, MDA) content in serum and liver and (Glutathione, GSH) level in brain were determined. The results show that in comparison with the control, the SOD activity in serum and liver increased significantly in groups received less than 0.75Gy, but de- creased obviously in groups irradiated to 0.75Gy or larger doses; the MDA content in serum and liver decreased sig- nificantly in groups of 0.3Gy or less doses, but increased obviously in groups of 0.3Gy or larger doses; the GSH level in brain tissue increased in groups of less than 0.5Gy, but decreased in groups of greater than 0.5Gy. Therefore, the low dose 12C6 ions irradiation can trigger off the antioxidant system in multi-tissue of mice, while the high doses irradiation can impair the antioxidant system.