淡紫拟青霉固态发酵黄芩药渣工艺优化

为了降低淡紫拟青霉（Paeciloids lilacinus）的固态发酵成本，以黄芩药渣废弃物为基质，对其培养基组成及发酵条件进行优化，并考察不同几丁质含量对菌株产几丁质酶活性的影响。结果表明，淡紫拟青霉固态发酵的最佳培养基组成为黄芩药渣20 g，玉米粉0.8 g，尿素0.02 g，含水量80%，固态发酵最佳的培养条件为培养温度28℃，接种量10%，培养时间7 d，在此优化条件下，分生孢子数可达8.98×109 CFU/g。添加0.9%几丁质时，可显著增加菌株产几丁质酶酶活性（P<0.05）。

Optimization of solid-state fermentation process of Scutellaria baicalensis residue by Paecilomyces lilacinus

In order to reduce the cost of solid-state fermentation of Paeciloids lilacinus, using Scutellaria baicalensis residue as the substrate, the medium composition and fermentation conditions were optimized, and the effects of different chitin content on chitinase-producing activity were investigated. The results showed that the optimal medium composition for solid-state fermentation of P. lilacinus was as follows: S. baicalensis residue 20 g, corn flour 0.8 g, urea 0.02 g, and water content 80%. The optimum culture conditions of solid-state fermentation were as follows: culture temperature 28 ℃, inoculum 10%, and time 7 d. Under the optimal condition, the conidia number could reach 8.98×109 CFU/g. The chitinase-producing activity of the strain was significantly increased when the chitin addition was 0.9% (P＜0.05).