产褐藻胶裂解酶弧菌HB161653的产酶条件优化

为提高弧菌HB161653产褐藻胶裂解酶活性，该研究通过单因素和正交试验对弧菌HB161653的产酶条件进行了优化。结果表明，最优的发酵培养基组成为海藻酸钠5 g/L，蛋白胨7.5 g/L，NaCl 25 g/L，K2HPO4 0.2 g/L，MgSO4 0.1 g/L；最优发酵条件为培养基初始pH 7.0，接种量1.5%，发酵温度28 ℃，发酵周期12 h。在此优化产酶条件下，褐藻胶裂解酶活力可达42.1 U/mL，较优化前（26.0 U/mL）提高了62%。该研究可为弧菌HB161653产褐藻胶裂解酶的规模化生产和褐藻寡糖的制备提供理论依据。

Optimization of enzyme production conditions for alginate lyase-producing Vibrio sp. HB161653

In order to improve the activity of alginate lyase produced by Vibrio sp. HB161653, the enzyme production conditions were optimized by single-factor and orthogonal tests. The results showed that the optimal fermentation medium was as follow: sodium alginate 5 g/L, peptone 7.5 g/L, NaCl 25 g/L, K2HPO4 0.2 g/L, MgSO4 0.1 g/L, and the optimal fermentation conditions were initial pH of the medium 7.0, inoculum 1.5%, temperature 28 ℃, and time 12 h. Under these optimized enzyme production conditions, the activity of alginate lyase reached 42.1 U/ml, which was 62% higher than that before optimization (26.0 U/ml). This study provided a theoretical basis for the large-scale production of alginate lyase produced by Vibrio sp. HB161653 and the preparation of alginate oligosaccharides.