基于响应面法优化谷氨酸棒杆菌发酵条件

该研究以谷氨酸棒杆菌（Corynebacterium glutamicum）P169为研究对象，以谷氨酸产量为主要评价指标，采用单因素试验和响应面法对其发酵条件进行优化，并进行摇瓶和20 L罐分批补料发酵验证。结果表明，谷氨酸棒杆菌P169产谷氨酸的最佳发酵条件为酵母粉41.0 g/L、葡萄糖27.0 g/L、尿素12.0 g/L和pH 7.0。在此优化条件下，谷氨酸产量达25.1 g/L，比优化前（16.5 g/L）提高了52.1%。以此为基料进行20 L罐分批补料发酵，谷氨酸产量达155 g/L，比优化前（142 g/L）提高了9.2%。该研究为提高谷氨酸棒杆菌谷氨酸产量提供了一种技术解决方案。

Optimization of Corynebacterium glutamate fermentation conditions based on response surface methodology

Using Corynebacterium glutamicum P169 as research object, glutamic acid production as main evaluation index, the fermentation conditions were optimized by single-factor test and response surface methodology, and the fermentation conditions were verified by shake flask and 20 L tank batch replenishment. The results showed that the optimal fermentation conditions for glutamic acid production by C. glutamicum P169 were as follows: yeast powder 41.0 g/L, glucose 27.0 g/L, urea 12.0 g/L and pH 7.0. Under the optimal conditions, glutamic acid yield reached to 25.1 g/L, which was 52.1% higher than that before optimization (16.5 g/L). Based on the medium, 20 L tank batch replenishment fermentation was conducted, and glutamic acid yield reached to 155 g/L, which was 9.2% higher than that before optimization (142 g/L). The study provided a technical solution for improving the glutamate yield of C. glutamate.