| 三江镇腌菜中降解亚硝酸盐乳酸菌的筛选和初步鉴定 |
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| 引用本文： | 刘志文,袁伟静,张三燕,罗静,陈忠良,李昆太,郭晓燕,徐波.三江镇腌菜中降解亚硝酸盐乳酸菌的筛选和初步鉴定[J].食品科学,2012,33(1):166-169. |
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| 作者姓名： | 刘志文 袁伟静 张三燕 罗静 陈忠良 李昆太 郭晓燕 徐波 |
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| 作者单位： | 江西农业大学生物科学与工程学院南昌市发酵应用技术重点实验室;北京市立新学校;南方医科大学第二临床医学院 |
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| 基金项目： | 江西省教育厅科研基金项目(GJJ11407);南昌市科技局科研基金项目(洪科发计字(2011)158-52);江西省科技厅科技支撑项目(2009BNB05704) |
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| 摘 要： | 为筛选具有较强降解亚硝酸盐能力的乳酸菌,本实验经过初筛和复筛从三江镇雪里蕻腌菜中筛选出降解亚硝酸盐能力相对较强的乳酸菌菌株。筛选结果显示菌株对亚硝酸盐最强降解能力达到68h平均降解量32.66μg/mL。采用十六烷基三甲基溴化铵法(CTAB)法提取降解亚硝酸盐能力最强的乳酸菌11号菌株的基因组,测序后进行Blast同源性比较,其与Lactobacillus fermentum strain PL9005的同源性达到98%,并选取同源性较高的菌株构建系统发育进化树,初步鉴定该菌株为Lactobacillus fermentum。
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| 关 键 词： | 乳酸菌 亚硝酸盐降解 筛选 鉴定 |
Screening and Preliminary Identification of a Lactic Acid Bacterium Strain with the Capacity of Nitrite Degradation from Sanjiang Pickled Potherb Mustard |
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| LIU Zhi-wen,YUAN Wei-jing,ZHANG San-yan,LUO Jing,CHEN Zhong-liang,LI Kun-tai,GUO Xiao-yan,XU Bo.Screening and Preliminary Identification of a Lactic Acid Bacterium Strain with the Capacity of Nitrite Degradation from Sanjiang Pickled Potherb Mustard[J].Food Science,2012,33(1):166-169. |
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| Authors: | LIU Zhi-wen YUAN Wei-jing ZHANG San-yan LUO Jing CHEN Zhong-liang LI Kun-tai GUO Xiao-yan XU Bo |
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| Affiliation: | 1,(1.Nanchang Key Laboratory of Applied Fermentation Technology,College of Bioscience and Engineering,Jiangxi Agricultural University,Nanchang 330045,China;2.The Lixin College of Beijing,Beijing 100037,China; 3.Second Clinical Medical College of Southern Medical University,Guangzhou 510515,China) |
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| Abstract: | A strain of lactic acid bacteria with strong ability to degrade nitrite was screened from pickled potherb mustard made in Sanjiang town,Jiangxi province.The strongest ability of the strain to degrade nitrite averagely reached 32.66 μg/mL during 68 h of fermentation.The results of 16s rRNA sequencing showed that this strain was 98% homologous to Lactobacillus fermentum PL9005.Polygenetic analysis indicated that this strain belonged to Lactobacillus fermentum. |
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| Keywords: | lactic acid bacteria nitrite degradation screening identification |
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