Cytotoxicity of fipronil on mice liver cells |
| |
Authors: | De Oliveira Patrícia Rosa Bechara Gervásio Henrique Denardi Sandra Eloisi Oliveira Rodrigo Juliano Mathias Maria Izabel Camargo |
| |
Affiliation: | 1. Departamento de Biologia, Instituto de Biociências, UNESP, 13506‐900 Rio Claro, SP, Brazil;2. Departamento de Patologia Veterinária, FCAV, UNESP, Via de Acesso Prof. Paulo Castellane, 14884‐900 Jaboticabal, SP, Brazil;3. Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, UEL, Brazil |
| |
Abstract: | In the present study, mice livers were examined following exposure to different doses of fipronil (15, 25, and 50 mg/kg). Histological and histochemical techniques were used to determine the cytotoxic potential of this compound and to assess the damage it caused to livers. Mice were divided into four groups: control group and groups I, II, and III were exposed to 15, 25, and 50 mg/kg fipronil, respectively. Our findings revealed cytological, morphohistological, and histochemical alterations in liver cells of animals from groups I, II, and III compared to group control animals. These changes included Kupffer-cell proliferation, hepatocyte hypertrophy, accumulation and distribution of proteins, polysaccharides, lipids, and vacuoles in the cytoplasm of hepatocytes, and congestion of blood vessels. These phenotypes mainly characterize the following: (a) autophagic processes, (b) steatosis, and (c) cell death by necrosis, which demonstrate the damage caused by fipronil on nontarget organisms in artificial conditions. |
| |
Keywords: | acaricide autophagy histopathology pesticide toxicity |
本文献已被 PubMed 等数据库收录! |
|