百合乌药蒲公英汤对I型糖尿病肝病改善和保健作用机制研究

目的评价百合乌药蒲公英汤(DecotionofBaiheWuyaoPugongying,DBWP)对I型糖尿病(type1diabetes,T1D)肝病的改善作用,并进行机制探讨。方法8周龄雄性昆明小鼠腹腔注射链脲佐菌素(streptozotocin,STZ)制备T1D模型,模型制备成功后随机将小鼠分为7组,随后用不同剂量DBWP连续灌胃6周进行效果评价。然后观察小鼠肝组织形态学变化和纤维化状况;检测肝功能、空腹血糖和血清胰岛素水平;检测血清中抗氧化因子含量;同时检测肝脏炎症因子、抗氧化因子和糖异生因子在mRNA水平的表达。结果DBWP给药后,与模型组相比,给药组小鼠体重有所上升,血糖值降低,血清胰岛素水平升高,以中剂量组效果最为明显;染色结果可见肝细胞排列整齐紧密,肝脏病理损伤有所恢复;血清生化相关酶含量明显降低,且以低1剂量最为明显;给药组抗氧化因子超氧化物歧化酶(superoxidedismutase,SOD)中、低1剂量组明显上升、丙二醛(malondialdehyde,MDA)含量以中、高剂量下降明显,自由基清除能力增强,整体药效以中剂量效果最为显著。通过对DBWP组中剂量组进行检测,结果发现,与模型组相比,给药组肝脏中炎症相关因子在mRNA水平表达减少,抗炎抗氧化水平增强,DBWP可降低肝脏中糖原合成因子的表达,糖异生抑制明显。结论DBWP通过减轻炎症、增加抗氧化能力来改善血糖,减轻T1D及其并发肝损伤。

Research on the mechanism of Decotion of Baihe Wuyao Pugongying on improving and health care of type I diabetic liver disease

Objective To evaluate the ameliorative effect of the Decotion of Baihe Wuyao Pugongying (DBWP) on type 1 diabetes (T1D) and liver disease, and explore its mechanism. Methods Eight-week-old male Kunming mice were intraperitoneally injected with streptozotocin (STZ) to establish the T1D model, after the model was successfully established, the mice were randomly divided into 7 groups and then gavage with different doses of DBWP for 6 weeks to evaluate the efficacy. Then the morphological changes and fibrosis of mouse liver were observed; liver function, fasting blood glucose and serum insulin levels were detected; the content of antioxidant factors in serum; the mRNA levels of inflammatory factors, antioxidant factors and gluconeogenesis factors in the liver were also detected. Results After administration of DBWP, compared with the model group, the body weight of the mice in the administration group was increased, the blood glucose level was decreased, and the serum insulin level was increased, with the effect in the medium-dose group being the most significant; the staining results showed that the hepatocytes were arranged neatly and tightly, and the pathological injury of the liver was restored; serum biochemical-related enzyme levels were significantly decreased, and the most obvious performance was in the low dose group 1; the antioxidant factor superoxide dismutase (SOD) in the administration group was significantly increased in the middle and low dose group 1, the malondialdehyde (MDA) content was significantly decreased in the middle and high dose groups, and the free radical scavenging ability was enhanced, and the effect of the drug was most pronounced in the middle dose. The medium-dose group of DBWP was tested, and the results showed that compared with the model group, the mRNA expression levels of inflammation-related factors in the liver of the administration group were decreased, and the anti-inflammatory and anti-oxidant levels were enhanced, DBWP could reduce the expression of glycogen synthesis factors in the liver, and gluconeogenesis was significantly inhibited. Conclusion DBWP can improve blood sugar and alleviate T1D and its complicated liver injury by reducing inflammation and increasing antioxidant capacity.