灰树花多肽-钙螯合物对小鼠衰老性骨质疏松的改善作用

通过建立D-半乳糖致衰老性骨质疏松(SOP)小鼠模型,探讨灰树花多肽-钙螯合物(GPs-Ca)对SOP的改善作用;通过体外Caco-2单细胞层模型模拟研究灰树花多肽-钙螯合物的促钙吸收能力.将50只雄性ICR小鼠随机分为5组,除了空白对照组外,其它4组均给予腹腔注射D-半乳糖;空白对照组给予腹腔注射同等剂量的生理盐水....

Improving Effects of Grifola frondosa Polypeptide-calcium Chelate on Senile Osteoporosis in Mice

Through the establishment of D-galactose-induced senile osteoporosis(SOP)mouse model,the effect of Grifola frondosa polypeptide-calcium chelate(GPs-Ca)on SOP was explored.The calcium absorption promoting ability of Grifola frondosa polypeptide-calcium chelate was studied by Caco-2 single cell layer model in vitro.Fifty male ICR mice were randomly divided into 5 groups.Except for the blank control group,the other four groups were given intraperitoneal injection of D-galactose;the control group was given intraperitoneal injection of the same dose of normal saline.After 8 weeks,the model was successfully established.Both the control group and the model control group were given deionized water intragastrically;the treated group was given a high dose of Grifola frondosa polypeptide-calcium chelate(Hgps-Ca).After 4 weeks of continuous treated in each group,blood was collected to detect the blood biochemical indicators,and the femur and tibia were stripped to determine the bone indicators.A Caco-2 monolayer was established,and Fluo-3-Am calcium ion probe was used to measure intracellular calcium uptake.Serum calcium and phosphorus levels in the Hgps-Ca group increased significantly,with statistical significance(P<0.05);serum alkaline phosphate(ALP)values of each group were lower than those in the model group,and the serum ALP level of Hgps-Ca group was close to that of the control group.GPs-Ca was more easily absorbed and transported in cells than inorganic calcium.The results showed that Grifola frondosa polypeptide-calcium chelate can improve serum calcium,phosphorus,CAT levels,reduce ALP activity,promote calcium salt deposition,improve calcium absorption and utilization rate,which is conducive to the treatment of SOP.