玉米须中高含量植物甾醇的提纯工艺

以干玉米须为原料,研究其植物甾醇的提纯结晶工艺。皂化处理后的玉米须的提取条件经过优化后,在超声波功率220 W、液料比12∶1（m L/g）、35℃超声处理55 min的条件下,玉米须总甾醇得率达到10.5886 mg/g。得到的粗品经氯仿萃取后,以乙酸乙酯为提取溶剂得到结晶,再滴加甲醇抽滤后得到甾醇重结晶。其中,β-谷甾醇和豆甾醇的纯度分别为47.5%±0.24%和36.7%±0.13%,植物甾醇纯度为92.76%。 

Process of extraction and purification of high content phytosterol from corn silk

The extraction and purification process of phytosterol were studied from corn silk by saponification. The highest extraction yield of 10.5886 mg / g total phytosterol was acquired by the ultrasonic assisted method. The optimal extraction conditions were determined by single factor and orthogonal test as follows: 220 W ultrasonic power,55 min extraction time,ultrasonic temperature 35 ℃,and 12∶ 1 ratio of liquld- material( m L / g).Phytosterol crystal was achieved with ethyl acetate as solvent after being extracted by chloroform.The purity of the crude phytosterols was 92.76% with 47.5% ± 0.24% β- sitosterol and 36.7% ± 0.13% campesterol,respectively after recrystallation by methanol.