Concanavalin A immobilized magnetic poly(glycidyl methacrylate) beads for antibody purification |
| |
Authors: | Birnur Akkaya Handan Yavuz Ferda Candan Adil Denizli |
| |
Affiliation: | 1. Department of Chemistry, Cumhuriyet University, Sivas, Turkey;2. Department of Chemistry, Hacettepe University, Ankara, Turkey |
| |
Abstract: | Concanavalin A (Con A) immobilized magnetic poly(glycidyl methacrylate) (mPGMA) beads in monosize and spherical for (1.62 μm in diameter) were used for the purification of human immunoglobulin G (IgG) from human plasma. Con A was immobilized by covalent binding onto the mPGMA beads. The maximum IgG adsorption on the mPGMA-Con A beads was observed at pH 6.0. The nonspecific IgG adsorption onto the plain mPGMA beads was very low (0.22 mg/g). Scatchard analysis of the adsorption isotherm for IgG on mPGMA-Con A beads showed an affinity constant (Ka) of 1.39 × 105 M?1 and a theoretical maximum adsorption capacity of 109.1 mg/g. An apparent IgG adsorption capacity of 66.2 mg/g was observed under the experimental conditions. IgG adsorption capacity from human plasma was observed as 48.0 mg/g. The adsorption of human serum albumin from plasma was 2.0 mg/g. The total protein adsorption was determined to be 50.0 mg/g. IgG molecules could be repeatedly adsorbed and eluted with the mPGMA-Con A beads without noticeable loss in the IgG adsorption capacity. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012 |
| |
Keywords: | magnetic beads separation techniques chromatography lectin affinity chromatography concanavalin A IgG |
|
|