丝瓜瓤固定黄孢原毛平革菌对2,4-二氯酚的降解研究

利用植物载体丝瓜瓤对黄孢原毛平革菌进行固定,研究了固定化细胞对2,4-二氯酚的降解性能。利用固定化细胞流化床反应器连续处理质量浓度为20mg/L的2,4-二氯酚废水。考察了不同水力停留时间对处理效果的影响。结果表明,采用吸附生长法能有效实现黄孢原毛平革菌的固定,固定细胞量可达0.791g细胞]/丝瓜瓤]。固定化细胞最适降解温度和pH值分别为35℃、6.0。对于质量浓度在20mg/L以下的低浓度2,4-二氯酚,固定化细胞和游离细胞的降解速率相当;对于质量浓度在50～120mg/L的高浓度2,4-二氯酚,固定化细胞具有明显优势,不仅可以耐受更高浓度的2,4-二氯酚,其降解速率也高于游离细胞,最大降解速率是13.95mg/(L·d),是游离细胞的2倍。不同的水力停留时间对固定化细胞流化床反应器连续处理2,4-二氯酚的降解效率有很大影响,固定化细胞对2,4-二氯酚降解过程遵循Monod方程。

Biodegradation of 2,4-DCP by Phanerochaete chrysosporium immobilized on luffa cylindrical sponge

Phanerochaete chrysosporium was immobilized on luffa cylindrical sponge and the biodegradation performance of immobilized cells on 2,4-DCP was studied. Using immobilized cells fluidized bed reactor to continu-ously treat 2,4-DCP wastewater whose mass concentration was 20 mg/L, the influence of HRT on the treatment effect was investigated. The results showed that, the immobilization of Phanerochaete chrysosporium could be realized throu-gh the adsorption growth, the immobilized cells amount reached 0.791 gcells]/gluffa cylindrical sponge]. The optimal temperature and pH for cells immobilization were 35 ℃ and 6.0 respectively. For the 2,4-DCP wastewater whose mass concentration was blow 20 mg/L, the biodegradation rate by the immobilized cells was equal to that by the free cells; however, for the said kind of wastewater whose mass concentration was 50 - 120 mg/L, immobilized cells showed prominent advantages, for they could bear higher concentration 2,4-DCP and obtained better biodegra-dation rate, the highest biodegradation rate reached 13.95 mg/(L·d), which was twice as that by the free cells. HRT had a great influence on the biodegradation rate of 2,4-DCP continuously treated by immobilized cells fluidized bed reactor and the process of immobilized cells biodegrading 2,4-DCP was found to follow the Monod equation.